PARP1-Trap Agarose is an affinity reagent for immunoprecipitation of human PARP1.
It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.
Human PARP1, does NOT bind to PARP2, 3, and 9
Immunoprecipitation (IP) / Co-IP
On-bead enzyme assays
|Product PARP1-Trap Agarose||Size 250 µL (10 reactions)||Code xta-10||Price $ 275||Buy +|
|Product PARP1-Trap Agarose||Size 500 µL (20 reactions)||Code xta-20||Price $ 475||Buy +|
|Product PARP1-Trap Agarose||Size 2.5 mL (100 reactions)||Code xta-100||Price $ 2050||Buy +|
|Product PARP1-Trap Agarose||Size 5 mL (200 reactions)||Code xta-200||Price Please inquire|
|Product PARP1-Trap Agarose||Size 10 mL (400 reactions)||Code xta-400||Price Please inquire|
|Product PARP1-Trap Agarose Kit||Size 500 µL (20 reactions)||Code xtak-20||Price $ 595||Buy +|
|Product Binding Control Agarose Beads||Size 500 µL (20 reactions)||Code bab-20||Price $ 75||Buy +|
|Product Spin Columns||Size 10 units||Code sct-10||Price $ 35||Buy +|
|Product Spin Columns||Size 20 units||Code sct-20||Price $ 60||Buy +|
|Product Spin Columns||Size 50 units||Code sct-50||Price $ 130||Buy +|
|Product PARP1-Binding-Protein||Size 250 µL||Code xt-250||Price Please inquire|
Epitope: Within DNA binding domain of PARP1
Does NOT bind to PARP 2, 3, and 9
Coupled Nanobody/ VHH
Recombinant, monoclonal anti-PARP1 single domain antibody (sdAb) fragment
Bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Storage buffer: 20 % EtOH
- SDS sample buffer
- 0.2 M glycine pH 2.5
Instead of elution, we recommend on-bead assays like on-bead digestion for MS analysis.
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year
How can I avoid unspecific protein interactions binding to the trap?
How can I elute bound proteins from a trap in their native state?
You can elute your fusion protein of interest with 0.2 M glycine pH 2.5 at room temperature. Pipette the beads up and down for 60-120 seconds and repeat this step. Ensure to neutralize your supernatant immediately afterwards by adding 1 M Tris base pH 10.4.
How many mammalian cells are required for an immunoprecipitation reaction?
For one immunoprecipitation reaction, we recommend using ~10^6 - 10^7 mammalian cells. The yield is also dependent on the expression level of your protein of interest and the interaction partners.
How much cell extract should I use for an immunoprecipitation reaction?
For other type of cells than mammalian cells, we recommend using 0.5 - 1.0 mg of cell extract.
Do I need to elute bound proteins from the beads for mass spectrometry analysis?
Do I need to elute my protein of interest from the beads for enzymatic assays?
What is the amount of trap slurry I need for one immunoprecipitation reaction?
25 µL slurry are sufficient for one pull-down reaction as the affinity of the traps is very high.
PARP1-Trap Agarose Kit
The PARP1-Trap Agarose is also available in a kit, including:
- PARP1-Trap Agarose
- Lysis*, wash and elution buffers
*The lysis buffer provided is suitable for mammalian cells. For other cell types use another suitable lysis buffer.
Binding Control for Agarose Beads
Only for research applications, not for diagnostic or therapeutic use!