Nano-Secondaries

10
Description
Nano-Secondaries® are a novel class of secondary antibodies for higher resolution & cleaner images. They consist of Nanobodies/ VHHs that bind to primary antibodies with high affinity in a species and subtype specific manner. Nano-Secondaries are conjugated to Alexa Fluor® dyes.
The next level of secondary antibodies
Higher resolution in immunofluorescence
Fast one-step immunostaining
No Cross-reactivity to IgG of other frequently used species and subtypes
Applications
Immunofluorescence
Super-resolution microscopy
Western blotting
Flow cytometry
Nano-Secondaries
Nano-Secondary alpaca anti-human IgG
Nano-Secondary alpaca anti-mouse IgG1
Nano-Secondary alpaca anti-mouse IgG2b
Nano-Secondary alpaca anti-mouse IgG3
Nano-Secondary alpaca anti-rabbit IgG
Brochure
Nano-Secondaries product brochure (PDF)
Better images with Nanobodies (PDF)
Nano-Secondary alpaca anti-human IgG
Code | Description | Size | Price | Qty |
---|---|---|---|---|
Code srbAF488-1-10 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] | Size 10 µL | Price $ 100 | |
Code srbAF488-1-100 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] | Size 100 µL | Price $ 365 | |
Code srbAF568-1-10 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 [CTK0101, CTK0102] | Size 10 µL | Price $ 100 | |
Code srbAF568-1-100 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 [CTK0101, CTK0102] | Size 100 µL | Price $ 365 | |
Code srbAF647-1-10 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 [CTK0101, CTK0102] | Size 10 µL | Price $ 100 | |
Code srbAF647-1-100 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 [CTK0101, CTK0102] | Size 100 µL | Price $ 365 | |
Code srbGCys2-1-500 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, for 2x Cys conjugation [CTK0101] | Size 500 μg | Price Please inquire |
Nano-Secondary alpaca anti-mouse IgG1
Code | Description | Size | Price | Qty |
---|---|---|---|---|
Code sms1AF488-1-10 | Description Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] | Size 10 µL | Price $ 100 | |
Code sms1AF488-1-100 | Description Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104] | Size 100 µL | Price $ 365 | |
Code sms1AF568-1-10 | Description Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 568 [CTK0103, CTK0104] | Size 10 µL | Price $ 100 | |
Code sms1AF568-1-100 | Description Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 568 [CTK0103, CTK0104] | Size 100 µL | Price $ 365 | |
Code sms1AF647-1-10 | Description Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647 [CTK0103, CTK0104] | Size 10 µL | Price $ 100 | |
Code sms1AF647-1-100 | Description Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647 [CTK0103, CTK0104] | Size 100 µL | Price $ 365 | |
Code smsG1Cys2-1-500 | Description Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, for 2x Cys conjugation [CTK0103] | Size 500 μg | Price Please inquire | |
Code smsG1Cys2-2-500 | Description Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, for 2x Cys conjugation [CTK0104] | Size 500 μg | Price Please inquire |
Nano-Secondary alpaca anti-mouse IgG2b
Code | Description | Size | Price | Qty |
---|---|---|---|---|
Code sms2bAF488-1-10 | Description Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 488 [CTK0105, CTK0106] | Size 10 µL | Price $ 100 | |
Code sms2bAF488-1-100 | Description Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 488 [CTK0105, CTK0106] | Size 100 µL | Price $ 365 | |
Code sms2bAF568-1-10 | Description Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 568 [CTK0105, CTK0106] | Size 10 µL | Price $ 100 | |
Code sms2bAF568-1-100 | Description Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 568 [CTK0105, CTK0106] | Size 100 µL | Price $ 365 | |
Code sms2bAF647-1-10 | Description Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 647 [CTK0105, CTK0106] | Size 10 µL | Price $ 100 | |
Code sms2bAF647-1-100 | Description Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, Alexa Fluor® 647 [CTK0105, CTK0106] | Size 100 µL | Price $ 365 | |
Code smsG2bCys2-1-500 | Description Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, for 2x Cys conjugation [CTK0105] | Size 500 μg | Price Please inquire | |
Code smsG2bCys2-2-500 | Description Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, for 2x Cys conjugation [CTK0106] | Size 500 μg | Price Please inquire |
Nano-Secondary alpaca anti-mouse IgG3
Code | Description | Size | Price | Qty |
---|---|---|---|---|
Code sms3AF647-1-10 | Description Nano-Secondary® alpaca anti-mouse IgG3, recombinant VHH, Alexa Fluor® 647 [CTK0107] | Size 10 µL | Price $ 100 | |
Code sms3AF647-1-100 | Description Nano-Secondary® alpaca anti-mouse IgG3, recombinant VHH, Alexa Fluor® 647 [CTK0107] | Size 100 µL | Price $ 365 |
Nano-Secondary alpaca anti-rabbit IgG
Code | Description | Size | Price | Qty |
---|---|---|---|---|
Code srbAF488-1-10 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] | Size 10 µL | Price $ 100 | |
Code srbAF488-1-100 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 488 [CTK0101, CTK0102] | Size 100 µL | Price $ 365 | |
Code srbAF568-1-10 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 [CTK0101, CTK0102] | Size 10 µL | Price $ 100 | |
Code srbAF568-1-100 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 568 [CTK0101, CTK0102] | Size 100 µL | Price $ 365 | |
Code srbAF647-1-10 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 [CTK0101, CTK0102] | Size 10 µL | Price $ 100 | |
Code srbAF647-1-100 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, Alexa Fluor® 647 [CTK0101, CTK0102] | Size 100 µL | Price $ 365 | |
Code srbGCys2-1-500 | Description Nano-Secondary® alpaca anti-human IgG/anti-rabbit IgG, recombinant VHH, for 2x Cys conjugation [CTK0101] | Size 500 μg | Price Please inquire | |
Code srbGCys2-2-500 | Description Nano-Secondary® alpaca anti-rabbit IgG, recombinant VHH, for 2x Cys conjugation [CTK0102] | Size 500 μg | Price Please inquire |
One-step immunostaining protocol saves time
ChromoTek’s Nano-Secondaries consist of monovalent Nanobodies with very high affinity. Hence, they enable one-step immunostaining: the simultaneous incubation of primary antibody and Nano-Secondary.
This method saves incubation time and washing steps. Simultaneous incubation also supports:
- multiplexing,
- tissue penetration, and
- cell staining for flow cytometry.
Of course, Nano-Secondaries can be used according to standard immunostaining protocols following a sequential incubation of primary and Nano-Secondary, too. In sequential staining, you can simply substitute the Nano-Secondary for any secondary antibody.
Better imaging
- Better tissue penetration and small epitope-label displacement for higher resolution
- High sensitivity: detection of low expressed epitopes via primary antibody
- High affinity reduces background
No Cross-reactivity to IgGs of other frequently used species
The cross reactivity of the Nano-Secondary has been carefully tested. It shows no cross-reactivity to IgG & serum of frequently used species. Because of their high specificity our Nano-Secondaries do not require any kind of pre-adsorption.
Precise and reproducible staining
Nano-Secondaries enable very precise and reproducible stainings. In detail, they:
- bind in a site-specific manner to primary IgG antibodies
- are stoichiometrically labeled with Alexa Fluor dyes per VHH respective Nanobody
- are fully characterized and validated
- are recombinantly produced, high lot-to-lot consistency
Nano-secondaries are is totally animal free manufactured, though VHHs respective Nanobodies are generated from immunization of alpacas and llamas.
Only for research applications, not for diagnostic or therapeutic use!