Nano-CaptureLigand mouse IgG2a

Description
The Nano-CaptureLigand mouse IgG2a captures and immobilizes mouse IgG2a antibodies and Fc-fragments to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to the mouse IgG2a subclass with high affinity and in a site-directed manner.

Specificity: Fc-fragment of mouse IgG2a antibodies
Does not bind to: Goat, human, macaque, and sheep serum and to mouse IgG1, IgG2b, and IgG3
Cross-reactivity to mouse IgG2c
Nano-CaptureLigands specificity overview

Conjugates: Biotin

Applications
Mouse IgG2a capture to biosensors used in BLI and SPR
Mouse IgG2a immobilization for ELISA

Advanced immobilization of antibodies
Specific, stable, and site-directed immobilization of non-biotinylated mouse IgG2a antibodies to streptavidin/avidin

 

Product Size Code Price Buy
Product Nano-CaptureLigand™ mouse IgG2a, Fc-specific VHH, biotinylated Size 10 µL Code smsG2aB-1-10 Price $ 130
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Product Nano-CaptureLigand™ mouse IgG2a, Fc-specific VHH, biotinylated Size 100 µL Code smsG2aB-1-100 Price $ 480
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Product Type
Capture Nanobody (VHH)

Applications
Immobilization of mouse IgG2a antibodies to avidin and streptavidin surfaces for Bio-Layer Interferometry (BLI), Surface Plasmon Resonance (SPR), and ELISA

Target/ Specificity 
Fc-fragment of mouse IgG2a

Cross-reactivity
No cross-reactivity to mouse IgG1, Ig2b, IgG3; human IgG1, IgG2, IgG3, IgG4, IgA; rat IgG1, IgG2a, IgG2b; sera from goat, human, macaque (cynomolgus monkey), sheep
Cross-reactivity to mouse IgG2c

Affinity (Kd) of monovalent (1:1) binding mode
1.5 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).

Concentration 
1 g/L (68 μM)

Conjugate
Biotin

Degree of biotinylation 
On average 1-2 biotin molecules per Nanobody

Format 
Alpaca single domain antibody, monovalent

Host
Alpaca-derived, recombinantly produced in bacteria

Clonality
Monoclonal

Clone
CTK0108 (VHH0249)

How to cite this product
Nano-CaptureLigand™ mouse IgG1, Fc-specific VHH, biotinylated
RRID: AB_2848188

Synonyms 
Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Validation 
Application validated for ELISA and BLI (FortéBio Octet® systems)
Determination of cross-reactivity, subclass specificity, sequence, affinity, and melting temperature

Purity
Recombinantly expressed and purified via His-tag

Form
Buffered aqueous solution

Storage buffer 
25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide

Storage conditions 
Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F

Stability
Stable for 1 year at +4°C/+40°F

Shipment 
Shipped at ambient temperature

Applications

  • Detection and quantitation of mouse IgG2a antibodies and their antigens
  • Characterization of mouse IgG2a antibodies’ binding kinetics and affinity
  • Mouse IgG2a antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma
  • Epitope binning

The next level of antibody immobilization to BLI and SPR biosensors

  • Gentle, site-directed and Fc-specific immobilization of mouse IgG2a antibodies and Fc-fragments to streptavidin coated biosensors without biotinylation of the antibody
  • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)
  • High affinity for stable baseline without significant dissociation
  • >10x regeneration for multiple reuse
  • High specificity enables mouse IgG2a antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

 

Comparison of BLI/SPR immobilization methods

The next level of antibody immobilization for ELISA

  • Compatible to enzyme-linked immunosorbent assays (ELISA) 
  • Site-directed immobilization of mouse IgG2a capture antibodies to streptavidin/avidin coated surfaces of sandwich ELISAs without biotinylation of antibody
  • Combine the site-specificity of protein A, G, and L with the stability of the biotin/streptavidin interaction
  • Mouse IgG2a subclass specificity allows the application of a broad range of detection antibodies 
  • High affinity for strong immobilization of mouse IgG2a antibodies even at low antibody concentrations

 

Comparison of ELISA immobilization methods

Nano-CaptureLigands are comprehensively validated

  • Characterized: DNA and protein sequences, affinity, melting temperature
  • Recombinantly produced, virtually without lot-to-lot variation
  • Manufacturing process is animal free

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Only for research applications, not for diagnostic or therapeutic use!