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Nano-Boosters & Nano-Labels

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Size comparison between Nano-Booster/ Nano-Label (fluorescently labeled Nanobody) and IgG complex consisting of primary and secondary antibody
Schematics of Nano-Booster application: The signals of GFP- and RFP-fusion protein decrease after cell fixation. The addition of GFP-/ RFP-Booster enhances the signals.

ChromoTek's Nano-Boosters & Nano-Labels are fluorescent probes. They consist of very small recombinant antibody domains, also called Nanobodies or VHHs. These single-domain alpaca antibody fragments are covalently coupled to fluorescent dyes.

  • The Nano-Boosters GFP- and RFP-Boosters enhance the signal of fluorescent proteins
  • The Nano-Labels Vimentin- and Histone-Label fluorescently label endogenous cellular proteins
  • The Spot-Label fluorescently labels the Spot-Tag, a 12-aa peptide tag

Applications
Immunofluorescence (IF): immunocytochemistry (ICC) and immunohistochemistry (IHC)
Cleared tissue, organ, and animal (DISCO) staining for light-sheet microscopy imaging
Super-resolution microscopy (SRM)

GFP-Booster

ORDER GFP-Booster
IF SRM GFP
x
Code Description Size Price Qty
Code gba488-10 Description GFP-Booster conjugated to ATTO488 Size 10 µL Price $ 100
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Code gba488-100 Description GFP-Booster conjugated to ATTO488 Size 100 µL Price $ 365
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Code gba594-10 Description GFP-Booster conjugated to ATTO594 Size 10 µL Price $ 100
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Code gba594-100 Description GFP-Booster conjugated to ATTO594 Size 100 µL Price $ 365
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Code gba647n-10 Description GFP-Booster conjugated to ATTO647N Size 10 µL Price $ 110
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Code gba647n-100 Description GFP-Booster conjugated to ATTO647N Size 100 µL Price $ 395
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Code gb2AF647-10 Description GFP-Booster conjugated to Alexa Fluor® 647 Size 10 µL Price $ 170
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Code gb2AF647-50 Description GFP-Booster conjugated to Alexa Fluor® 647 Size 50 µL Price $ 395
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Code gbas635p-10 Description GFP-Booster conjugated to Abberior AS 635P Size 10 µL Price $ 100
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Code gbas635p-100 Description GFP-Booster conjugated to Abberior AS 635P Size 100 µL Price $ 365
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Code gt-250 Description GFP VHH, recombinant binding protein Size 250 µL Price $ 275
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Code custom Description GFP-Binding protein, custom labeling Size Price Please inquire

Histone-Label

ORDER Histone-Label
IF SRM
x
Code Description Size Price Qty
Code tba488-10 Description Histone-Label conjugated to Atto488 Size 10 µL Price $ 100
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Code tba488-100 Description Histone-Label conjugated to Atto488 Size 100 µL Price $ 365
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Code tt-250 Description Histone-Binding-protein, 1 mg/mL Size 250 µL Price $ 275
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RFP-Booster

ORDER RFP-Booster
IF SRM
x
Code Description Size Price Qty
Code rba594-10 Description RFP-Booster conjugated to ATTO594 Size 10 µL Price $ 100
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Code rba594-100 Description RFP-Booster conjugated to ATTO594 Size 100 µL Price $ 365
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Code rba647n-10 Description RFP-Booster conjugated to ATTO647N Size 10 µL Price $ 110
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Code rba647n-100 Description RFP-Booster conjugated to ATTO647N Size 100 µL Price $ 395
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Code rb2AF647-10 Description RFP-Nanobody conjugated to Alexa Fluor® 647 Size 10 µL Price $ 170
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Code rb2AF647-50 Description RFP-Nanobody conjugated to Alexa Fluor® 647 Size 50 µL Price $ 395
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Code rt-250 Description RFP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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Code custom Description RFP-Binding protein, custom labeling Size Price Please inquire

Spot-Label

ORDER Spot-Label
IF SRM Spot WB
x
Code Description Size Price Qty
Code eba488-10 Description Spot-Label conjugated to Atto488 Size 10 µL Price $ 100
Qty +
Code eba488-50 Description Spot-Label conjugated to Atto488 Size 50 µL Price $ 395
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Code eba594-10 Description Spot-Label conjugated to Atto594 Size 10 µL Price $ 100
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Code eba594-50 Description Spot-Label conjugated to Atto594 Size 50 µL Price $ 395
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Vimentin-Label

ORDER Vimentin-Label
IF SRM
x
Code Description Size Price Qty
Code vba488-10 Description Vimentin-Label conjugated to Atto488 Size 10 µL Price $ 100
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Code vba488-100 Description Vimentin-Label conjugated to Atto488 Size 100 µL Price $ 365
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Why size matters

Nano-Boosters & Nano-Labels (also termed fluorophore-conjugated Nanobodies) are much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to their small size, VHHs are probes for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

Benefits

  • Higher image resolution and higher labelling density
  • Considerably higher tissue penetration rates
  • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments
  • Less than 2 nm epitope-label displacement minimizes linkage error
  • Stabilization, enhancement and reactivation of fluorescent signals
  • Monovalent VHHs do not cluster their epitopes
  • Consistent and reliable performance due to recombinant production and monoclonality
  • Validated antibodies: structure and function characterized

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Only for research applications, not for diagnostic or therapeutic use!