Nano-Boosters & Nano-Labels

Size comparison between Nano-Booster/ Nano-Label (fluorescently labeled Nanobody) and IgG complex consisting of primary and secondary antibody

Schematics of Nano-Booster application: The signals of GFP- and RFP-fusion protein decrease after cell fixation. The addition of GFP-/ RFP-Booster enhances the signals.

Immunostaining with GFP-Booster Alexa Fluor 488 (green) and RFP-Booster Alexa Fluor 568 (red). HeLa cells were transiently transfected with Tom70-eGFP and PCNA-mRFP. Scale bar, 10 µm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

ChromoTek's Nano-Boosters & Nano-Labels are fluorescent probes. They consist of very small recombinant antibody domains, also called Nanobodies or V_HHs. These single-domain alpaca antibody fragments are covalently coupled to fluorescent dyes.

The Nano-Boosters GFP- and RFP-Boosters enhance the signal of fluorescent proteins
The Nano-Labels Vimentin- and Histone-Label fluorescently label endogenous cellular proteins
The Spot-Label fluorescently labels the Spot-Tag, a 12-aa peptide tag

Applications
Immunofluorescence (IF): immunocytochemistry (ICC) and immunohistochemistry (IHC)
Cleared tissue, organ, and animal (DISCO) staining for light-sheet microscopy imaging
Super-resolution microscopy (SRM)

Product Listing
Applications

GFP-Booster

PRICE GFP-Booster

IF SRM GFP

Code	Description	Size	Price	Qty
Code gb2AF488-10	Description GFP-Booster conjugated to Alexa Fluor® 488	Size 10 µL	Price $ 170	Qty + +
Code gb2AF488-50	Description GFP-Booster conjugated to Alexa Fluor® 488	Size 50 µL	Price $ 395	Qty + +
Code gb2AF568-10	Description GFP-Booster conjugated to Alexa Fluor® 568	Size 10 µL	Price $ 170	Qty + +
Code gb2AF568-50	Description GFP-Booster conjugated to Alexa Fluor® 568	Size 50 µL	Price $ 395	Qty + +
Code gb2AF647-10	Description GFP-Booster conjugated to Alexa Fluor® 647	Size 10 µL	Price $ 170	Qty + +
Code gb2AF647-50	Description GFP-Booster conjugated to Alexa Fluor® 647	Size 50 µL	Price $ 395	Qty + +
Code gba488-10	Description GFP-Booster conjugated to ATTO488	Size 10 µL	Price $ 100	Qty + +
Code gba488-100	Description GFP-Booster conjugated to ATTO488	Size 100 µL	Price $ 365	Qty + +
Code gba594-10	Description GFP-Booster conjugated to ATTO594	Size 10 µL	Price $ 100	Qty + +
Code gba594-100	Description GFP-Booster conjugated to ATTO594	Size 100 µL	Price $ 365	Qty + +
Code gba647n-10	Description GFP-Booster conjugated to ATTO647N	Size 10 µL	Price $ 110	Qty + +
Code gba647n-100	Description GFP-Booster conjugated to ATTO647N	Size 100 µL	Price $ 395	Qty + +
Code gt-250	Description GFP VHH, recombinant binding protein	Size 250 µL	Price $ 275	Qty + +
Code custom	Description GFP-Binding protein, custom labeling	Size	Price Please inquire

Histone-Label

PRICE Histone-Label

IF SRM

Code	Description	Size	Price	Qty
Code tba488-10	Description Histone-Label conjugated to Atto488	Size 10 µL	Price $ 100	Qty + +
Code tba488-100	Description Histone-Label conjugated to Atto488	Size 100 µL	Price $ 365	Qty + +
Code tt-250	Description Histone-Binding-protein, 1 mg/mL	Size 250 µL	Price $ 275	Qty + +

RFP-Booster

PRICE RFP-Booster

IF SRM

Code	Description	Size	Price	Qty
Code rb2AF568-10	Description RFP-Nanobody conjugated to Alexa Fluor® 568	Size 10 µL	Price $ 170	Qty + +
Code rb2AF568-50	Description RFP-Nanobody conjugated to Alexa Fluor® 568	Size 50 µL	Price $ 395	Qty + +
Code rb2AF647-10	Description RFP-Nanobody conjugated to Alexa Fluor® 647	Size 10 µL	Price $ 170	Qty + +
Code rb2AF647-50	Description RFP-Nanobody conjugated to Alexa Fluor® 647	Size 50 µL	Price $ 395	Qty + +
Code rba594-10	Description RFP-Booster conjugated to ATTO594	Size 10 µL	Price $ 100	Qty + +
Code rba594-100	Description RFP-Booster conjugated to ATTO594	Size 100 µL	Price $ 365	Qty + +
Code rba647n-10	Description RFP-Booster conjugated to ATTO647N	Size 10 µL	Price $ 110	Qty + +
Code rba647n-100	Description RFP-Booster conjugated to ATTO647N	Size 100 µL	Price $ 395	Qty + +
Code rt-250	Description RFP-Binding-Protein, 1 mg / mL	Size 250 µL	Price $ 275	Qty + +
Code custom	Description RFP-Binding protein, custom labeling	Size	Price Please inquire

Spot-Label

PRICE Spot-Label

IF SRM Spot WB

Code	Description	Size	Price	Qty
Code eba488-10	Description Spot-Label conjugated to Atto488	Size 10 µL	Price $ 100	Qty + +
Code eba488-50	Description Spot-Label conjugated to Atto488	Size 50 µL	Price $ 395	Qty + +
Code eba594-10	Description Spot-Label conjugated to Atto594	Size 10 µL	Price $ 100	Qty + +
Code eba594-50	Description Spot-Label conjugated to Atto594	Size 50 µL	Price $ 395	Qty + +

Vimentin-Label

PRICE Vimentin-Label

IF SRM

Code	Description	Size	Price	Qty
Code vba488-10	Description Vimentin-Label conjugated to Atto488	Size 10 µL	Price $ 100	Qty + +
Code vba488-100	Description Vimentin-Label conjugated to Atto488	Size 100 µL	Price $ 365	Qty + +

Why size matters

Nano-Boosters & Nano-Labels (also termed fluorophore-conjugated Nanobodies) are much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to their small size, V_HHs are probes for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

Benefits

Higher image resolution and higher labelling density
Considerably higher tissue penetration rates
Superior accessibility and labelling of epitopes in crowded cellular/organelle environments
Less than 2 nm epitope-label displacement minimizes linkage error
Stabilization, enhancement and reactivation of fluorescent signals
Monovalent V_HHs do not cluster their epitopes
Consistent and reliable performance due to recombinant production and monoclonality
Validated antibodies: structure and function characterized

Only for research applications, not for diagnostic or therapeutic use!