DYKDDDDK Fab-Trap

Description
ChromoTek DYKDDDDK Fab-Trap™ for immunoprecipitation of DYKDDDDK-tagged proteins, also known as Flag®-tagged proteins. The DYKDDDDK Fab-Trap comprises an anti-DYKDDDDK Fab-fragment coupled to agarose beads.

Specificity
The DYKDDDDK Fab-Trap binds to the 1x and 3x DYKDDDDK sequence (1x and 3xFlag®-tag) at the N-terminus, C-terminus, or internal site of the fusion protein.

Applications
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)
Protein purification
ChIP/ RIP analysis
On-bead enzyme assays

Formats/matrices
DYKDDDDK Fab-Trap Agarose

Try out the DYKDDDDK IP Starter Pack (ffap)
DYKDDDDK Fab-Trap™ Agarose (20 reactions, product code ffa-20) for immunoprecipitation, 3xDYKDDDDK-peptide (1mg, fp-1) for elution and DYKDDDDK tag Polyclonal antibody (Binds to FLAG® tag epitope) (20 µL, 20543-1-AP-20UL) for detection in Western blots.

Benefit from DYKDDDDK Fab-Trap for immunoprecipitation (IP)

  • Lowest background in class
  • Less contaminating antibody chains 
  • Recombinantly expressed Fab-fragment derived from M2 clone
  • Compatible with harsh buffer conditions
  • Ready-to-use 
  • Highly selective binding of N-or C-terminal Flag-tag position 

DYKDDDDK Immunoprecipitation Starter Pack

The DYKDDDDK Immunoprecipitation Starter Pack (product ffap) includes

Format and Matrix

We offer the DYKDDDDK Fab-Trap coupled to Agarose beads: DYKDDDDK Fab-Trap Agarose

  DYKDDDDK Fab-Trap Agarose
matrix Agarose
color white
particle size 90 µm
magnetic no
may be centrifuged up to 2,500 x g

In addition, we offer a DYKDDDDK Fab-Trap Agarose Kit including DYKDDDDK Fab-Trap Agarose, lysis for mammalian cells , wash and elution buffers.

Stable & reliable binding

  • Compatible to stringent wash conditions such as  2 M NaCl, 10 mM DTT, 2 M Urea
  • Applications validated and characterized

About DYKDDDDK-tag

The DYKDDDDK-tag, also called Flag-tag, is an artificial epitope. It is not derived from a natural protein and the amino acid  sequence is DYKDDDDK. The DYKDDDDK-tag is used to tag proteins for immunoprecipitation, protein purification, Western blotting, and other capture and detection applications. The DYKDDDDK-tag is used in bacteria, yeast, insect and mammalian cell systems either as 1xDYKDDDDK-tag or 3xDYKDDDDK-tag (1x and 3xFlag®-tag).

 

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Only for research applications, not for diagnostic or therapeutic use!