Chromobodies

A Chromobody is a genetic fusion of a target-specific VHH from alpaca and a fluorescent protein (TagRFP or TagGFP, EGFP). The Chromobody is available in form of a plasmid or selected stable cell lines (not shown).

The nanoprobe Chromobody is an intracellularly expressed fusion protein, which consists of a target-specific Nanobody and a fluorescent protein.

Time lapse video of Actin-Chromobodies expressed in HeLa cells, which have been exposed to Cytochalasin D treatment for 1 hour and then recovered for 4 hours.

Cell Cycle-Chromobody expressed in HeLa cells. The time lapse video shows cell division. The signal of TagRFP has been imaged.

Time lapse video of Vimentin-Chromobodies expressed in HeLa cells. The signal of TagGFP has been imaged.

Intracellular Functional Antibodies or Intrabodies

ChromoTek’s Chromobodies^® are fluorescent nanoprobes for real-time and live-cell imaging of intracellular endogenous proteins.
A Chromobody is a small intracellular functional antibody. It consists of a V_HH/ Nanobody that is genetically fused to a fluorescent protein. Unlike conventional antibodies, Chromobodies visualize endogenous cellular structures and processes in live cells and real-time. Chromobody probes label non-invasively their targets without interfering with target function. This makes them perfect nanoprobes for cellular research and high content analysis.
Chromobody products comprise plasmids and selected stable cell lines.
The Chromobody technology is covered by granted and pending international patents.
Learn more about our Chromobody technology

Product Listing
Applications

Actin-Chromobody

ORDER Actin-Chromobody plasmids

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code acr	Description Actin-Chromobody® plasmid (TagRFP)	Size 20 µg	Price $ 999	Qty + +
Code acg	Description Actin-Chromobody® plasmid (TagGFP2)	Size 20 µg	Price $ 999	Qty + +
Code acg-n	Description Nuclear Actin-Chromobody® plasmid (TagGFP2)	Size 20 µg	Price $ 999	Qty + +

Cell Cycle-Chromobody

ORDER Cell Cycle-Chromobody plasmid

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code ccr	Description Cell Cycle-Chromobody® plasmid (TagRFP)	Size 20 µg	Price $ 999	Qty + +

ORDER Cell Cycle-Chromobody TagGFP2 HeLa cell line

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code h-ccc-g	Description Frozen cells in FCS free cryopreservation reagent CryoMaxx	Size 5x 10^6 frozen cells	Price Please inquire

ORDER Cell Cycle-Chromobody TagGFP2 U2OS cell line

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code u-ccc-g	Description Frozen cells in FCS free cryopreservation reagent CryoMaxx	Size 5x 10^6 frozen cells	Price Please inquire

ORDER Cell Cycle-Chromobody TagRFP HeLa cell line

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code h-ccc-r	Description Frozen cells in FCS free cryopreservation reagent CryoMaxx	Size 5x 10^6 frozen cells	Price Please inquire

ORDER Cell Cycle-Chromobody TagRFP U2OS cell line

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code u-ccc-r	Description Frozen cells in FCS free cryopreservation reagent CryoMaxx	Size 5x 10^6 frozen cells	Price Please inquire

Dnmt1-Chromobody

ORDER Dnmt1-Chromobody plasmids

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code dcg	Description Dnmt1-Chromobody® plasmid (TagGFP2)	Size 20 µg	Price $ 999	Qty + +
Code dcr	Description Dnmt1-Chromobody® plasmid (TagRFP)	Size 20 µg	Price $ 999	Qty + +

Histone-Chromobody

ORDER Histone-Chromobody plasmid

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code tcg	Description Histone-Chromobody® plasmid (EGFP)	Size 20 µg	Price $ 999	Qty + +

Lamin-Chromobody

ORDER Lamin-Chromobody plasmid

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code lcg	Description Lamin-Chromobody plasmid (TagGFP2)	Size 20 µg	Price $ 999	Qty + +

PARP1-Chromobody

ORDER PARP1-Chromobody plasmids

HCA Live cell SRM

Code	Description	Size	Price	Qty
Code xcg	Description PARP1-Chromobody® plasmid (TagGFP2)	Size 20 µg	Price $ 999	Qty + +
Code xcr	Description PARP1-Chromobody® plasmid (TagRFP)	Size 20 µg	Price $ 999	Qty + +

Vimentin-Chromobody

ORDER Vimentin-Chromobody plasmid

HCA IF Live cell SRM

Code	Description	Size	Price	Qty
Code vcg	Description Vimentin-Chromobody® plasmid (TagGFP2)	Size 20 µg	Price $ 999	Qty + +

High content analysis

High-content screening (HCS) comprises multiple cellular assays that are conducted in an automated, parallel manner to analyze a set of different biological parameters in live cells in a high throughput format. It is used in cell biology and drug discovery to find compounds that impact cellular processes, protein-protein interactions, and/or the phenotype of cells. Tested compounds include small molecules, peptides, and RNAi. Assays are usually based on fluorescence imaging. Next to automated microscopes for data acquisition also automated image analysis software is used, because huge data amounts are generated. HCS is also called high-content analysis (HCA) or cellomics.

PRODUCTS

All Chromobodies are carefully selected to not interfere with endogenous protein function and offer the unique possibility to trace native proteins in living cells.

Only for research applications, not for diagnostic or therapeutic use!