RFP-Trap

Description

The ChromoTek RFP-Trap® is a ready to use affinity resin for immunoprecipitation (IP) of red fluorescent-fusion proteins like mCherry.

RFP-Trap consists of an anti-RFP Nanobody/ VHH coupled to agarose or magnetic agarose beads. ChromoTek’s RFP-Trap is referenced in more than 100 scientific articles.

Specificity
mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives, see:
Fluorescent protein specificity table

Applications
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)
On-bead enzyme assays
ChIP / RIP analysis

Formats/matrices
RFP-Trap Agarose
RFP-Trap Magnetic Agarose
RFP VHH, recombinant binding protein

Synonyms
anti-RFP VHH, RFP binding protein, RFP Nanobody, or anti-RFP single domain antibody fragment (sdAb)

Benefit from RFP-Trap for Immunoprecipitation (IP)

  • Reliable and robust pull-down of mCherry and other RFP-fusion proteins
  • From cell to gel in less than one hour
  • High affinity
  • Strong binding even under harsh washing conditions
  • No heavy & light chains in your downstream application

Format/matrices & properties

We offer the RFP binding protein coupled to agarose beads and magnetic agarose beads,  or uncoupled as purified protein.

 RFP-Trap agaroseRFP-Trap magnetic agarose
matrixagarosemagnetic agarose
colorwhiteblack
particle size90 µm40 µm
binding capacity8 µg/ 10 µL8 µg/ 10 µL
magneticnoyes
may be centrifuged up to2,500 x g800 x g

Extraordinary stable & reliable binding

  • Dissociation constant KD of 5 nM
  • Compatible to harsh wash conditions: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
  • Fully applications validated and characterized

Specificity

The RFP-Trap specifically binds to

  • mCherry, mOrange
  • mRFP, mRFPruby, mPlum, tagRFP, mKate2, mScarlet

See full fluorescent protein specificity list here.

About RFP

The first red fluorescent protein (RFP) that became commercially available is DsRed. It was isolated from Discosoma sp in 1999. Additional RFPs have been isolated from Anthozoa. Site directed mutagenesis was used to create RFP derivatives with fluorescence in orange, red, and far red colors. Also, monomerized versions were generated and brightness, maturation efficiency, and photostability were improved, making theses RFPs valuable research tools.

 

Brochure

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Only for research applications, not for diagnostic or therapeutic use!