Myc-tag antibody [9E1]

Description
Rat monoclonal [9E1] to Myc-tag

Specificity
The antibody recognizes the Myc-tag sequence EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein.

Applications
Western Blot: 1:1,000
Immunofluorescence: 1:500
ELISA: 1:1,000
Immunoprecipitation: 5 µg/ IP

ProductSizeCodePriceBuy
ProductMyc-tag antibody [9E1]Size20 µLCode9e1-20Price $ 100
Buy+
ProductMyc-tag antibody [9E1]Size100 µLCode9e1-100Price $ 245
Buy+

Isotype
IgG1

Host species & clonality
Rat monoclonal

Form
Purified antibody

Size
20 μL; 100 μL

Purity
Protein G purified

Protein concentration
1.1 mg/mL

Storage buffer
PBS, preservative: 0.09 % Sodium azide

RRID
AB_2631398

Storage instructions
Shipped on blue ice. Upon receipt store at 4°C. Stable for one year.

Do not freeze

Does the Myc-tag antibody [9E1] bind endogenous c-Myc protein?

We could not detect binding of the Myc-tag antibody [9E1] to endogenous c-Myc protein. Some epitope residues that have shown to be crucial for binding to the Myc-tag antibody are buried in the three-dimensional structure of the c-Myc protein. Hence, under native conditions, c-Myc protein is not a suitable binding partner for the Myc-tag antibody [9E1].

Can I use the Myc-tag antibody [9E1] for immunoprecipitation?

Yes, you can use the Myc-tag antibody [9E1] for immunoprecipitation and immobilize it on beads through protein A or G.

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Only for research applications, not for diagnostic or therapeutic use!