Dnmt1-Chromobody

Novel nanoprobe to trace Dnmt1 in live cells non-invasively

The Dnmt1-Chromobody® visualizes endogenous Dnmt1 in real-time during cell cycle progression.

The Dnmt1-Chromobody comprises the antigen binding domain (VHH) derived from an alpaca heavy chain antibody genetically fused to TagRFP or TagGFP2.

Specificity
Dnmt1, the major eukaryotic DNA methyltransferase; tested in human cells

Applications
Live cell imaging

Dnmt1-Chromobody products
Dnmt1-Chromobody plasmids

Benefits

  • Trace dynamic changes during the cell cycle in real time
  • Monitor the distribution of an endogenous cell cycle marker protein without overexpression artifacts or cytotoxic effects from overexpressed fluorescent protein fusion Dnmt1

About Dnmt1

 

DNA methylation is a crucial part of normal organismal development and cellular differentiation in higher organisms. DNA methylation stably alters the gene expression pattern in cells such that cells can "remember where they have been" or decrease gene expression; for example, cells programmed to be pancreatic islets during embryonic development remain pancreatic islets throughout the life of the organism without continuing signals telling them that they need to remain islets.

By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development.

Dnmt1-Chromobody Plasmid (TagGFP2)

Mammalian expression vector encoding major eukaryotic DNA methyltransferase Dnmt1-VHH fused to the green fluorescent protein TagGFP2 (Evrogen)

Dnmt1-Chromobody Plasmid (TagRFP)

Mammalian expression vector encoding major eukaryotic DNA methyltransferase Dnmt1-VHH fused to the red fluorescent protein TagRFP (Evrogen)

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Only for research applications, not for diagnostic or therapeutic use!