Novel nanoprobe to trace Dnmt1 in live cells non-invasively
The Dnmt1-Chromobody® visualizes endogenous Dnmt1 in real-time during cell cycle progression.
The Dnmt1-Chromobody comprises the antigen binding domain (VHH) derived from an alpaca heavy chain antibody genetically fused to TagRFP or TagGFP2.
Dnmt1, the major eukaryotic DNA methyltransferase; tested in human cells
Live cell imaging
- Trace dynamic changes during the cell cycle in real time
- Monitor the distribution of an endogenous cell cycle marker protein without overexpression artifacts or cytotoxic effects from overexpressed fluorescent protein fusion Dnmt1
DNA methylation is a crucial part of normal organismal development and cellular differentiation in higher organisms. DNA methylation stably alters the gene expression pattern in cells such that cells can "remember where they have been" or decrease gene expression; for example, cells programmed to be pancreatic islets during embryonic development remain pancreatic islets throughout the life of the organism without continuing signals telling them that they need to remain islets.
By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development.
Dnmt1-Chromobody Plasmid (TagGFP2)
Dnmt1-Chromobody Plasmid (TagRFP)
Only for research applications, not for diagnostic or therapeutic use!