ChromoTek’s proprietary and novel Spot-System is the first peptide-tag specific Nanobody for universal capture & detection applications. It comprises Spot-Tag^®, an inert 12 amino acid peptide-tag (PDRVRAVSHWSS), which has been affinity optimized, and the universal, rugged Spot-Nanobody that specifically binds to Spot-tagged proteins with high affinity.

In general, Nanobodies tend to bind to native, discontinuous conformational epitopes (3-dimensional epitopes) rather than to linear peptide epitopes (2-dimensional epitopes). However, ChromoTek has managed to develop exceptional anti-Spot-Tag V_HH next to anti-Myc V_HH, which recognize small linear peptide tags, respectively.