iST GFP-Trap Kit
The iST GFP-Trap Kit enables researchers to process GFP-fusion proteins and their interacting partners for Mass Spectrometry analysis by including the ChromoTek GFP-Trap for immunoprecipitation/Co-IP of GFP-fusion proteins and the PreOmics iST buffers and cartridges required for bottom-up proteomic sample preparation. This robust method yields purified peptides while dramatically reducing contamination and sample loss. Each kit accommodates up to eight samples and includes pull-down reagent for controls.
IP / Co-IP of GFP fusion proteins & sample preparation for MS
- Reliable identification of GFP-fusion proteins & interactomes
- Superior processing of IP samples into clean peptide mixtures
- Intuitive protocol for reproducible results
Make the most of your time & gain about 40 hours
The ChromoTek GFP-Trap is a Nanobody or VHH coupled to a matrix for immunoprecipitation (IP) of GFP-fusion proteins & their interacting partners.
- fast and efficient one-step IP
- more than 1,000 publications
- gold standard for IP of GFP fusion proteins
The PreOmics iST technology provides a complete solution for proteomic sample preparation.
- developed at the laboratory of Matthias Mann
- streamlined workflow directly compatible with MS analysis
- avoids MS downtime through clean peptide samples
MS-based proteomics typically employs multiple sample preparation steps that can lead to sample contamination and loss. The PreOmics iST technology overcomes these problems and has been published in Nature Methods (Kulak et al., 2014): “Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells” (doi: 10.1038/nmeth.2834).
The PreOmics iST technology includes all chemicals, enzymes, cartridges and plasticware required for denaturation, reduction, and alkylation of co-immunoprecipitated proteins plus in-solution digestion and peptide clean-up. Optimized and patented peptide washing procedures eliminate both hydrophobic and hydrophilic contaminants. This results in clean peptides, decreasing MS downtime and providing reproducible and reliable results. For more information see www.preomics.com.
|iST GFP-Trap Kit||8 (plus 2 controls)||gtak-iST-8|
The iST GFP-Trap Test Kit contains sufficient GFP-Trap_A beads for up to 10 immunoprecipitations. Out of these ten reactions, two shall be used for negative/positive controls by immunoblot analysis. The iST GFP-Trap Test Kit can be used for up to 8 iST proteomic sample preparation reactions.
What are the biophysical parameters of the GFP-Trap®?
Molecular weight: 14,1 kDa
Extinction coefficient: 27055 M-1 cm-1
Is there a size limit for GFP labeled structures that can bind to the beads of the GFP-Trap®?
Can I purify GFP labeled fusion proteins directly from tissue samples, i.e. in a denaturing buffer?
In principle the GFP-Trap® is very stable even under harsh buffer conditions (e.g. RIPA buffer containing 0.1% SDS or 1M urea).