Gold Standard

GFP-Trap®

The ChromoTek GFP-Trap® is the gold standard for fast, reliable and effective one-step immunoprecipitation of GFP-fusion proteins and their interacting factors from cell extracts or organelles.

Use GFP-Trap® for:

  • Immunoprecipitation (IP) / Co-IP
  • Mass spectrometry (MS)
  • Enzyme activity measurements
  • RIP analysis

Extraordinary stable & reliable binding

  • Dissociation constant KD of 1pM
  • Stable up to 80°C, 1mM DTT, 3M Guanidinium•HCl, 8M Urea, 2M NaCl, 2% Nonidet P40 Substitute, 1% SDS, 1% Triton X-100
  • Fulfills highest requirements for antibody validation
  • Structure and function are characterized

Specificity

The GFP-Trap® specifically binds to most common GFP derivatives:

  • AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K
  • CFP, eCFP, mCerulean
  • YFP, Citrine, eCitrine, eYFP, Venus, Ypet
  • BFP

"GFP-Trap® is the best beads ever!"

Dr. Kenkyo Matsuura, Department of Pharmacology and Cancer Biology, Duke University (find more references here >)

Immunoprecipitation GFP

Immunoprecipitations of GFP from human cells expressing GFP

Input (I), non-bound (FT) and bound (B) fractions were separated by SDS-PAGE followed by Coomassie staining and Western Blotting. The bound fraction of the conventional GFP antibodies shows in addition to GFP contaminating heavy chain and light chain. The bound fraction of the GFP-Trap® does not contain these contaminating polypeptide chains. Note effective binding of GFP-fusion proteins: No GFP-fusions left in non-bound (FT) lane in Western Blot.

Benefit from GFP-Trap® for Immunoprecipitation

  • Effective pulldown of GFP-fusion proteins for consistent results
  • No heavy & light antibody chains, short incubation (5-30 min)
  • Extraordinary binding, also under harsh conditions
  • Very high affinity (KD=1pM*) to bind even low abundant proteins
  • Reliable gold standard with more than 900 publications

"GFP-Trap® vs. Antibodies"

Download our latest white paper and get a quick overview on how you could benefit from alpaca Nano-Traps.

Download PDF

Try before you buy: Get a test sample of the GFP-Trap® - in return we just need you to send back your test results within 4 weeks.

 

Available matrices & properties

We offer the GFP-Trap® coupled to agarose beads, magnetic agarose beads, magnetic particles, and multiwell plates, or uncoupled as purified protein.

agarose beadsmagnetic agarose beadsmagnetic particles
matrixagaroseagarosesilica (non porous)
colourwhiteblackbrown
particle size90µm40µm0,5-1µm
binding capacity12µg/10µl8µg/10µl3-4µg/10µl
magneticnoyesyes
centrifuged up to 3000g3000g3000g 

*Kinetic parameters of the GFP-Trap have been measured using Dynamic Biosensors’ switchSENSE® technology.