Vimentin VHH
Description
Alpaca anti-Vimentin VHH, purified recombinant binding protein
Synonyms
Vimentin Binding-Protein, Vimentin-Nanobody, Vimenti-Label (unconjugated), and Vimentin single domain antibody (sdAb)
Specificity
Vimentin-Label specifically binds to the vimentin intermediate filament protein. Validated on canine MDCK cells, rodent BHK cells and on human A549 cells upon stimulation with TGFβ.
Applications
Immunofluorescence
Coupling of dyes, biotin, etc. via NHS Ester reaction
| Product | Size | Code | Price | Buy |
|---|---|---|---|---|
| Product Vimentin VHH, recombinant binding protein | Size 250 µL | Code vt-250 | Price $ 500 |
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Specificity
Vimentin intermediate filament protein
Conjugation:
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Biophysical properties
Molecular weight MW: 26.7 kDa
Molecular extinction coefficient ε: 48,150 L·Mol−1·cm−1
Isoelectrical point pI: 10 (theoretical)
Form
Purified recombinant antibody fragment, monoclonal, bivalent
Two anti-Vimentin VHHs/Nanobodies genetically fused via a linker
Protein concentration
1 mg/mL
Storage buffer
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
How to cite this product
RRID: AB_2827563
Storage instructions
Shipped on blue ice. Upon receipt store at 4°C; stable for one year.
Do not freeze.
Are the nanobodies monoclonal or polyclonal?
The nanobodies are recombinant/monoclonal.
What are the biophysical parameters of the Nanobodies?
| Name | MW (kDa) | Molar Extinction Coefficient (M-1 cm-1) | Theoretical pI |
|---|---|---|---|
| GFP VHH | 13.9 | 27.055 | 9.4 |
| GST VHH | 14.8 | 28.545 | 8.2 |
| Halo VHH | 14.8 | 23.045 | 9.4 |
| Histon VHH | 14.4 | 25.565 | 10.2 |
| MBP VHH | 14.1 | 27.055 | 10.0 |
| Mdm4/Hdmx VHH | 13.6 | 14.565 | 10.1 |
| MK2 VHH | 14.4 | 32.555 | 9.7 |
mNeonGreen VHH | 14.3 | 21.680 | 8.0 |
| Myc VHH | 13.5 | 23.045 | 9.6 |
| p53 C-term VHH | 14.8 | 34.170 | 9.2 |
| p53 N-term VHH | 13.0 | 20.065 | 9.7 |
| PARP1 VHH | 14.4 | 17.670 | 9.2 |
| RFP VHH | 14.9 | 30.035 | 9.3 |
| SNAP/CLIP-tag VHH | 13.1 | 24.075 | 9.8 |
| Spot VHH (bivalent) | 30.3 | 46.340 | 5.3 |
| TurboGFP VHH | 13.6 | 18.575 | 9.5 |
| Vimentin VHH | 26.7 | 48.150 | 10.0 |
Do the nanobodies have any tags?
Yes, the nanobodies have a C-terminal His6-tag.
Related products
Only for research applications, not for diagnostic or therapeutic use!