V5 VHH

Description
Alpaca anti-V5 VHH, purified recombinant binding protein

Synonyms
V5 Binding-Protein, V5 Nanobody, and V5-single domain antibody (sdAb)

Specificity
V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.

Applications
Coupling of  biotin etc. via NHS Ester reaction

Product Size Code Price Buy
Product V5 VHH, recombinant binding protein Size 250 µL Code v5t-250 Price Please inquire

Affinity
Dissociation constant KD of 40 nM

Specificity
V5-tag sequence motif GKPIPNPLLGLDST

Conjugation:
Unconjugated
Suitable for conjugation of biotin, beads, surfaces etc. via NHS ester reaction

Biophysical properties
Molecular weight MW: 14.6 kDa
Molecular extinction coefficient ε: 22,710 L·Mol−1·cm−1
Isoelectrical point pI: 8.0 (theoretical)

Form
Purified recombinant antibody fragment, monoclonal

Protein concentration
1 mg/mL

Storage buffer
25mM TAPS pH 8.5, 137 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide

How to cite this product
RRID: AB_2868499

Storage instructions
Shipped at ambient temperature . Upon receipt store at 4°C; stable for one year.

Do not freeze.

Are the nanobodies monoclonal or polyclonal?

The nanobodies are recombinant/monoclonal.

What are the biophysical parameters of the Nanobodies?

Name MW (kDa) Molar Extinction Coefficient
(M-1 cm-1)
Theoretical pI
GFP VHH 13.9 27,055 9.4
GST VHH 14.8 28,545 8.2
Halo VHH 14.8 23,045 9.4
Histon VHH 14.4 25,565 10.2
MBP VHH 14.1 27,055 10.0
Mdm4/Hdmx VHH 13.6 14,565 10.1
MK2 VHH 14.4 32,555 9.7
mNeonGreen VHH 14.3 21,680 8.0
Myc VHH 13.5 23,045 9.6
p53 C-term VHH 14.8 34,170 9.2
p53 N-term VHH 13.0 20,065 9.7
PARP1 VHH 14.4 17,670 9.2
RFP VHH 14.9 30,035 9.3
SNAP/CLIP-tag VHH 13.1 24,075 9.8
Spot VHH (bivalent) 30.3 46,340 5.3
TurboGFP VHH 13.6 18,575 9.5
V5 VHH 14.6 22,710 8.0
Vimentin VHH 26.7 48,150 10.0

Do the nanobodies have any tags?

Yes, the nanobodies have a C-terminal His6-tag.

Extraordinarily stable & reliable binding

  • Compatible to wash conditions such as 2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, or 4 M Urea
  • Dissociation constant KD of 40 nM
  • Applications validated and characterized

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Only for research applications, not for diagnostic or therapeutic use!