V5 VHH
1
Description
Alpaca anti-V5 VHH, purified recombinant binding protein
Synonyms
V5 Binding-Protein, V5 Nanobody, and V5-single domain antibody (sdAb)
Specificity
V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Applications
Coupling of biotin etc. via NHS Ester reaction
| Product | Size | Code | Price | Buy |
|---|---|---|---|---|
| Product V5 VHH, recombinant binding protein | Size 250 µL | Code v5t-250 | Price Please inquire |
Affinity
Dissociation constant KD of 40 nM
Specificity
V5-tag sequence motif GKPIPNPLLGLDST
Conjugation:
Unconjugated
Suitable for conjugation of biotin, beads, surfaces etc. via NHS ester reaction
Biophysical properties
Molecular weight MW: 14.6 kDa
Molecular extinction coefficient ε: 22,710 L·Mol−1·cm−1
Isoelectrical point pI: 8.0 (theoretical)
Form
Purified recombinant antibody fragment, monoclonal
Protein concentration
1 mg/mL
Storage buffer
25mM TAPS pH 8.5, 137 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
How to cite this product
RRID: AB_2868499
Storage instructions
Shipped at ambient temperature . Upon receipt store at 4°C; stable for one year.
Do not freeze.
Are the nanobodies monoclonal or polyclonal?
The nanobodies are recombinant/monoclonal.
What are the biophysical parameters of the Nanobodies?
| Name | MW (kDa) | Molar Extinction Coefficient (M-1 cm-1) | Theoretical pI |
|---|---|---|---|
| GFP VHH | 13.9 | 27,055 | 9.4 |
| GST VHH | 14.8 | 28,545 | 8.2 |
| Halo VHH | 14.8 | 23,045 | 9.4 |
| Histon VHH | 14.4 | 25,565 | 10.2 |
| MBP VHH | 14.1 | 27,055 | 10.0 |
| Mdm4/Hdmx VHH | 13.6 | 14,565 | 10.1 |
| MK2 VHH | 14.4 | 32,555 | 9.7 |
| mNeonGreen VHH | 14.3 | 21,680 | 8.0 |
| Myc VHH | 13.5 | 23,045 | 9.6 |
| p53 C-term VHH | 14.8 | 34,170 | 9.2 |
| p53 N-term VHH | 13.0 | 20,065 | 9.7 |
| PARP1 VHH | 14.4 | 17,670 | 9.2 |
| RFP VHH | 14.9 | 30,035 | 9.3 |
| SNAP/CLIP-tag VHH | 13.1 | 24,075 | 9.8 |
| Spot VHH (bivalent) | 30.3 | 46,340 | 5.3 |
| TurboGFP VHH | 13.6 | 18,575 | 9.5 |
| V5 VHH | 14.6 | 22,710 | 8.0 |
| Vimentin VHH | 26.7 | 48,150 | 10.0 |
Do the nanobodies have any tags?
Yes, the nanobodies have a C-terminal His6-tag.
Extraordinarily stable & reliable binding
- Compatible to wash conditions such as 2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, or 4 M Urea
- Dissociation constant KD of 40 nM
- Applications validated and characterized
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Only for research applications, not for diagnostic or therapeutic use!