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ChromoTek V5-Trap® beads for immunoprecipitation and purification of V5-tagged proteins. The V5-Trap comprises an anti-V5-tag Nanobody/ VHH coupled to Agarose, Magnetic Agarose, or Magnetic Particles M-270.

The V5-Trap binds to the V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.

Immunoprecipitation (IP) / Co-IP

Affinity purification
Mass spectrometry (MS)
On-bead enzyme assays
ChIP/ RIP analysis

V5-Trap Agarose
V5-Trap Magnetic Agarose
V5-Trap Magnetic Particle M-270
V5 VHH, recombinant binding protein
iST V5-Trap Kit for IP plus sample preparation for MS

V5 Nanobody, anti-V5 VHH, V5-binding protein or V5 single domain antibody fragment (sdAb)

Benefit from V5-Trap for Immunoprecipitation (IP)

  • No heavy & light antibody chains in downstream applications
  • Very low background
  • Effective elution with V5-peptide
  • Harsh washing conditions
  • Efficient immunoprecipitation of V5-tag fusion proteins

Format/matrices & properties

We offer the anti-V5 VHH coupled to Agarose beads, Magnetic Agarose beads, Magnetic Particles M-270 or uncoupled as purified protein.

  V5-Trap Agarose V5-Trap Magnetic Agarose V5-Trap Magnetic Particles M-270
matrix Agarose Magnetic Agarose Magnetic Particles M-270
color white black brown
particle size 90 µm 40 µm 2.8 µm
magnetic no yes yes
may be centrifuged up to 2,500 x g 800 x g 8,000 x g

Extraordinary stable & reliable binding

  • Dissociation constant KD of 40 nM
  • Compatible to harsh wash conditions such as  1 M NaCl, 2 % Nonidet P40 Substitute, 4 M Urea
  • Applications validated and characterized

About V5-tag

The V5-tag is derived from the P and V protein of the simian virus 5 (SV5, a paramyxovirus). The V5-tag is an often used peptide tag (epitope tag) to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems or to purify proteins. 

The amino acid sequence of the V5-tag is GKPIPNPLLGLDST.

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Only for research applications, not for diagnostic or therapeutic use!