ChromoTek V5-TrapTM beads for immunoprecipitation and purification of V5-tagged proteins. The V5-Trap comprises an anti-V5-tag Nanobody/ VHH coupled to agarose and magnetic agarose beads.
The V5-Trap binds to the V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)
On-bead enzyme assays
ChIP/ RIP analysis
V5 Nanobody, anti-V5 VHH, V5-binding protein or V5 single domain antibody fragment (sdAb)
Benefit from V5-Trap for Immunoprecipitation (IP)
- No heavy & light antibody chains in downstream applications
- Very low background
- Effective elution with V5-peptide
- Harsh washing conditions
- Efficient immunoprecipitation of V5-tag fusion proteins
Format/matrices & properties
We offer the anti-V5 VHH coupled to agarose beads and magnetic agarose beads, or uncoupled as purified protein.
|V5-Trap Agarose||V5-Trap Magnetic Agarose|
|particle size||90 µm||40 µm|
|may be centrifuged up to||2,500 x g||800 x g|
Extraordinary stable & reliable binding
- Dissociation constant KD of 40 nM
- Compatible to harsh wash conditions such as 1 M NaCl, 2 % Nonidet P40 Substitute, 4 M Urea
- Applications validated and characterized
The V5-tag is derived from the P and V protein of the simian virus 5 (SV5, a paramyxovirus). The V5-tag is an often used peptide tag (epitope tag) to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems or to purify proteins.
The amino acid sequence of the V5-tag is GKPIPNPLLGLDST.
Only for research applications, not for diagnostic or therapeutic use!