The ChromoTek V5-TrapTM Magnetic Agarose is an affinity resin for immunoprecipitation of V5-tagged proteins.
It consists of a anti V5 Nanobody/VHH coupled to magnetic agarose beads.
V5-tag amino acid sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.
ChIP/ RIP analysis
On-bead enzyme assays
|Product V5-Trap Magnetic Agarose||Size 250 µL (10 reactions)||Code v5tma-10||Price $ 275||Buy +|
|Product V5-Trap Magnetic Agarose||Size 500 µL (20 reactions)||Code v5tma-20||Price $ 475||Buy +|
|Product V5-Trap Magnetic Agarose||Size 2.5 mL (100 reactions)||Code v5tma-100||Price $ 2050||Buy +|
|Product V5-Trap Magnetic Agarose||Size 5 mL (200 reactions)||Code v5tma-200||Price Please inquire|
|Product V5-Trap Magnetic Agarose||Size 10 mL (400 reactions)||Code v5tma-400||Price Please inquire|
|Product V5-Trap Magnetic Agarose Kit||Size 500 µL (20 reactions)||Code v5tmak-20||Price $ 595||Buy +|
|Product V5-peptide||Size 1 mg||Code v5p-1||Price $ 85||Buy +|
|Product Binding Control Magnetic Agarose Beads||Size 500 µL (20 reactions)||Code bmab-20||Price $ 75||Buy +|
|Product V5 VHH, recombinant binding protein||Size 250 µL||Code v5t-250||Price Please inquire|
Dissociation constant KD of 40 nM
Wash buffer compatibility
2 % Nonidet P40 Substitute, 2 % Triton X-100, 1 M NaCl, 5 mM TCEP, 5 mM DTT, 10 mM ß-mercaptoethanol, 4 M Urea
V5-tag sequence motif GKPIPNPLLGLDST
10 µL slurry bind ~ 7 µg of recombinant V5-tagged protein ~30 kDa
Coupled Nanobody/ VHH
recombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment
Bead size: ~ 40 µm
Storage buffer: 20% EtOH
- V5-peptide. For details see below.
- SDS sample buffer
- 0.1 M glycine pH 2.0
Please consider whether elution is really needed. We recommend on-bead assays like on-bead digestion for MS analysis.
Compatibility with mass spectrometry
The V5-Trap is optimized for on-bead digestion (see protocol).
How to cite this product
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.
Should I use an N-terminal or C-terminal fusion tag?
Both the N-terminal or C-terminal fusion tag work well with the traps.
How can I avoid unspecific protein interactions binding to the trap?
How many mammalian cells are required for an immunoprecipitation reaction?
For one immunoprecipitation reaction, we recommend using ~10^6 - 10^7 mammalian cells. The yield is also dependent on the expression level of your protein of interest and the interaction partners.
How much cell extract should I use for an immunoprecipitation reaction?
For other type of cells than mammalian cells, we recommend using 0.5 - 1.0 mg of cell extract.
Do I need to elute bound proteins from the beads for mass spectrometry analysis?
Do I need to elute my protein of interest from the beads for enzymatic assays?
What is the amount of trap slurry I need for one immunoprecipitation reaction?
25 µL slurry are sufficient for one pull-down reaction as the affinity of the traps is very high.
- No heavy & light antibody chains
- Pull-down of V5-tagged proteins
- Harsh washing conditions
- Elution of native proteins
- One step immunoprecipitation
- Reproducible results
V5-Trap Magnetic Agarose Kit
The V5-Trap Magnetic Agarose is also available in a kit, including:
- V5-Trap Magnetic Agarose
- Lysis*, dilution and wash buffers
*The lysis buffer provided is suitable for mammalian cells. For other cell types use another suitable lysis buffer.
Binding Control for Magnetic Agarose Beads
Only for research applications, not for diagnostic or therapeutic use!