V5-tag antibody [SV5-P-K]

Western Blot (WB) analysis of His-eGFP-V5 added to HEK293T cell extract

The anti-V5 antibody [SV5-P-K] is a recombinant, monoclonal mouse IgG1, has very low background and detects even low amounts of V5-tagged proteins. Secondary antibody ChromoTek’s Alpaca anti-mouse IgG1 Alexa Fluor 488 Nano-Secondary.

Description
Mouse monoclonal [SV5-P-K] to V5-tag

Specificity
The antibody recognizes the V5-tag sequence motif GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.

Applications
Western Blot

Product	Size	Code	Price	Buy
Product V5-tag antibody [SV5-P-K]	Size 20 µL	Code v5ab-20	Price $ 100	Buy +
Product V5-tag antibody [SV5-P-K]	Size 200 µL	Code v5ab-200	Price $ 480	Buy +

Isotype
IgG1 kappa

Host species & clonality
Mouse monoclonal

Form
Purified antibody

Size
20 μL; 200 μL

Purity
Protein A purified

Protein concentration
1.0 mg/mL

Storage buffer
PBS, preservative: 0.02 % Proclin

How to cite this product
RRID: AB_2868500

Storage instructions
Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles.

V5-tag

The V5-tag is derived from the P and V protein of the simian virus 5 (SV5, a paramyxovirus), amino acid residues 95 to 108 of RNA polymerase alpha subunit. The V5-tag was first referenced in 1992¹.

¹Tomas Hanke, Paul Szawlowski and Richard E. Randall; Construction of solid matrix-antibody-antigen complexes containing simian immunodeficiency virus p27 using tag-specific monoclonal antibody and tag-linked antigen, Journal of General Virology (1992), 73, 653-660

Related products

Only for research applications, not for diagnostic or therapeutic use!