TurboGFP-Trap

Description
The TurboGFP-Trap is an affinity resin for immunoprecipitation of TurboGFP-fusion proteins.
It consists of an anti-TurboGFP Nanobody/ VHH, which is conjugated to beads.

Specificity
TurboGFP and CopGFP from the copepod Pontellina plumata.
The TurboGFP-Trap does not bind to jellyfish GFP and derivatives:  Fluorescent protein specificity table

Applications
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)
On-bead enzyme assays
ELISA

Formats/matrices:
TurboGFP-Trap Agarose
TurboGFP-Trap Magnetic Agarose
TurboGFP VHH, recombinant binding protein

Synonyms
anti-TurboGFP VHH, TurboGFP binding protein, TurboGFP Nanobody, or anti-TurboGFP single domain antibody fragment (sdAb)

Benefit from TurboGFP-Trap for Immunoprecipitation (IP)

  • Effective pull-down of TurboGFP fusion proteins, low background
  • NO heavy & light chain contamination
  • High binding affinity
  • Harsh washing conditions

Format/matrices & properties

We offer the TurboGFP VHH coupled to agarose beads and magnetic agarose beads, or uncoupled as purified protein.

  TurboGFP-Trap Agarose TurboGFP-Trap Magnetic Agarose
matrix agarose magnetic agarose
color white black
particle size 90 µm 40 µm
magnetic no yes
may be centrifuged up to 2,500 x g 800 x g

Extraordinary stable & reliable binding

  • Dissociation constant KD of 0.5 nM
  • Compatible to harsh wash conditions such as  6 M urea, 2 M NaCl
  • Applications validated and characterized

About TurboGFP

The dimeric green fluorescent protein TurboGFP is derived from the green fluorescent protein CopGFP of the copepod Pontellina plumata (Shagin et al., 2004). It possesses bright green fluorescence with excitation maximum at 482 nm and emission maximum at 502 nm. TurboGFP is a fast maturating protein: its fluorescent signal is visible earlier than other green fluorescent proteins. TurboGFP shares only about 20 % sequence identity with jellyfish GFP variants. Therefore, most anti-GFP antibodies including the GFP-Nanobody used in GFP-Trap do not bind to TurboGFP.

 

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Only for research applications, not for diagnostic or therapeutic use!