TurboGFP-Trap Magnetic Agarose

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.
It consists of an anti-TurboGFP VHH/ Nanobody that is coupled to magnetic agarose beads.

TurboGFP, CopGFP (P. plumata), maxGFP, maxFP-Green
The TurboGFP-Trap does not bind to jellyfish GFP and derivatives, see: Fluorescent protein specificity table

Immunoprecipitation/ Co-IP
Mass spectrometry
On-bead enzyme assays

Product Size Code Price Buy
Product TurboGFP-Trap Magnetic Agarose Size 250 µL (10 reactions) Code tbtma-10 Price $ 275
Buy +
Product TurboGFP-Trap Magnetic Agarose Size 500 µL (20 reactions) Code tbtma-20 Price $ 475
Buy +
Product TurboGFP-Trap Magnetic Agarose Size 2.5 mL (100 reactions) Code tbtma-100 Price $ 2050
Buy +
Product TurboGFP-Trap Magnetic Agarose Size 5 mL (200 reactions) Code tbtma-200 Price Please inquire
Product TurboGFP-Trap Magnetic Agarose Size 10 mL (400 reactions) Code tbtma-400 Price Please inquire
Product TurboGFP-Trap Magnetic Agarose Kit Size 500 µL (20 reactions) Code tbtmak-20 Price $ 595
Buy +
Product Binding Control Magnetic Agarose Beads Size 500 µL (20 reactions) Code bmab-20 Price $ 75
Buy +
Product TurboGFP VHH, recombinant binding protein Size 250 µL Code tbt-250 Price Please inquire

Dissociation constant KD of 0.5 nM

Wash buffer compatibility
6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS

TurboGFP, CopGFP, Pontellina plumata
maxGFP, maxFP-Green

Coupled Nanobody/ VHH
Recombinant, monoclonal anti-TurboGFP single domain antibody (sdAb) fragment

Bead properties
Bead size: ~ 40 µm
Storage buffer: 20 % EtOH

- SDS sample buffer
- 0.1 mM citrate acid pH 3.0
Please consider whether elution is really needed. We recommend on-bead assays like on-bead digestion for MS analysis.

Compatibility with mass spectrometry
The TurboGFP-Trap is optimized for on-bead digestion. Complete tryptic digest results in 5-6 peptides.

How to cite this product
RRID: AB_2827597

Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Does the GFP-Trap bind TurboGFP?

No, the GFP-Trap doesn't bind TurboGFP. TurboGFP is a green fluorescent protein derived from CopGFP of the copepod Pontellina plumata whereas GFP has been originally isolated from jellyfish Aequorea Victoria. Turbo-GFP shares only ~20 % sequence identity with the commonly used GFP variants.

Should I use an N-terminal or C-terminal fusion tag?

Both the N-terminal or C-terminal fusion tag work well with the traps.

How can I avoid unspecific protein interactions binding to the trap?

For preclearing of your sample we recommend to use our binding controls (bab-20 or bmab-20).

Please find more information in our Troubleshooting guide

How can I elute bound proteins from a trap in their native state?

You can elute your fusion protein of interest with 0.2 M glycine pH 2.5 at room temperature. Pipette the beads up and down for 60-120 seconds and repeat this step. Ensure to neutralize your supernatant immediately afterwards by adding 1 M Tris base pH 10.4.

How many mammalian cells are required for an immunoprecipitation reaction?

For one immunoprecipitation reaction, we recommend using ~10^6 - 10^7 mammalian cells. The yield is also dependent on the expression level of your protein of interest and the interaction partners.

How much cell extract should I use for an immunoprecipitation reaction?

For other type of cells than mammalian cells, we recommend using 0.5 - 1.0 mg of cell extract.

Do I need to elute bound proteins from the beads for mass spectrometry analysis?

No, you can directly conduct an on-bead digestion after immunoprecipitation. This procedure allows faster and more efficient sample preparation and a potential higher yield.
Please find more information here:

Preomics kit

Protocol on-bead digestion

Do I need to elute my protein of interest from the beads for enzymatic assays?

No, you can directly perform your enzymatic assay on the beads if the active center is not blocked.

Please find more information in our application note "enzymatic activity assay"

What is the amount of trap slurry I need for one immunoprecipitation reaction?

25 µL slurry are sufficient for one pull-down reaction as the affinity of the traps is very high.


  • Low background
  • NO heavy and light antibody chain contamination
  • High binding affinity
  • Harsh washing conditions

TurboGFP-Trap Magnetic Agarose Kit

The TurboGFP-Trap Magnetic Agarose is also available in a kit, including:

  • TurboGFP-Trap Magnetic Agarose
  • Lysis*, wash and elution buffers

*The lysis buffer provided is suitable for mammalian cells. For other cell types use another suitable lysis buffer.

Binding Control for Magnetic Agarose Beads

Download Whitepaper
Order Free Test-Sample
Email newsletter

Only for research applications, not for diagnostic or therapeutic use!