Affinity
Dissociation constant K_D of 6 nM

Specificity
Spot-Binding Protein recognizes the Spot-tag sequence motif PDRVRAVSHWSS at the N-terminus or C-terminus of the Spot-tagged protein

Conjugation:
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Spot VHH contains a sortase-tag and a His-tag at its C terminus: LPETGHHHHHH. This sequence includes the sortase recognition motif LPETG, for conjugation. In addition, bivalent Spot-VHH may be detected by an anti-His antibody. The Spot VHH corresponds to the “BC2-Nanobody” used by Virant et al in the publication "Nature Communications" (2018) doi:10.1038/s41467-018-03191-2.

Biophysical properties
Molecular weight MW: 30.3 kDa
Molecular extinction coefficient ε: 46,340 L·Mol⁻¹·cm⁻¹
Isoelectrical point pI: 5.3 (theoretical)

Form
Purified recombinant and bivalent antibody fragment.
Two anti-Spot-Tag VHHs/ Nanobodies genetically fused via a linker

Size
250 µL

Protein concentration
1 mg/mL

Storage buffer
25 mM TAPS pH8,5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide

RRID
AB_2827572

Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.

Do not freeze.

• Spot VHH, recombinant binding protein SDS (PDF)