Spot Vectors for cloning

Description
Spot-Tag vectors for cloning

Applications
Cloning your protein of interest (POI) to Spot-Tag
Expression of Spot-tagged fusion proteins

Product Size Code Price Buy
Product pSpot1 vector, E. coli, Spot-tag N-term., Kan., high expression Size 1.25 µg Code ev-1 Price $ 65
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Product pSpot2 vector, E. coli, Spot-tag C-term., Kan., high expression Size 1.25 µg Code ev-2 Price $ 65
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Product pSpot3 vector, E. coli, Spot-tag C-term., Amp., low expression Size 1.25 µg Code ev-3 Price $ 65
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Product pSpot4 vector, E. coli, Spot-tag N-term., Amp., low expression Size 1.25 µg Code ev-4 Price $ 65
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Product pSpot5 vector, S. cerevisiae, Spot-tag N-term., Leu, CEN, low expression Size 1.25 µg Code ev-5 Price $ 65
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Product pSpot6 vector, S. cerevisiae, Spot-tag C-term., Leu, CEN, low expression Size 1.25 µg Code ev-6 Price $ 65
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Product pSpot7 vector, S. cerevisiae, Spot-tag N-term., Leu, 2µ, high expression Size 1.25 µg Code ev-7 Price $ 65
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Product pSpot8 vector, S. cerevisiae, Spot-tag C-term., Leu, 2µ, high expression Size 1.25 µg Code ev-8 Price $ 65
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Spot vectors for cloning:

Vector Code Data-
sheet
Sequence file  SDS
 pSpot1 vector, E. coli, Spot-tag N-term., Kan., high expression  ev-1   ev-1.pdf   ev-1.gb   SDS
 pSpot2 vector, E. coli, Spot-tag C-term., Kan., high expression  ev-2   ev-2.pdf   ev-2.gb   SDS
 pSpot3 vector, E. coli, Spot-tag C-term., Amp., low expression  ev-3   ev-3.pdf   ev-3.gb   SDS
 pSpot4 vector, E. coli, Spot-tag N-term., Amp., low expression  ev-4   ev-4.pdf   ev-4.gb   SDS
 pSpot5 vector, S. cerevisiae, Spot-tag N-term., Leu, CEN, low expression   ev-5   ev-5.pdf   ev-5.gb   SDS
 pSpot6 vector, S. cerevisiae, Spot-tag C-term., Leu, CEN, low expression   ev-6   ev-6.pdf   ev-6.gb   SDS
 pSpot7 vector, S. cerevisiae, Spot-tag N-term., Leu, 2µ, high expression  ev-7   ev-7.pdf   ev-7.gb   SDS
 pSpot8 vector, S. cerevisiae, Spot-tag C-term., Leu, 2µ, high expression  ev-8   ev-8.pdf   ev-8.gb  SDS

Should I use an N-terminal or C-terminal Spot-fusions? Can I also insert the Spot-Tag in the middle of my protein?

Both, an N- or C-terminal fusion work well.
The use of the Spot-Tag for internal protein tagging has to be tested case by case. The Spot-Tag peptide has to exist in a linear form and be accessible without steric hindrance from other parts of the protein of interest. An internal Spot-Tag is only likely to be recognised by the Spot-Tag nanobody if inserted into a sufficiently large and unstructured loop, an inherently unstructured domain or a lengthy domain linker.

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Only for research applications, not for diagnostic or therapeutic use!