Spot-peptide for elution
Elution of Spot-tagged fusion proteins bound to Spot-Trap®.
Note: Optimal dilutions/ concentrations should be determined by the user
|Product Spot-peptide||Size 1 mg||Code ep-1||Price $ 85||Buy +|
Reconstitute Spot peptide in PBS to a final concentration of 2 mg/ml.
Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze / thaw cycles.
How can I elute bound Spot-tagged protein from the Spot-Trap in its native state?
You can elute your Spot-fusion competitively with Spot peptide. Alternatively, you can use 10 mM NaOH pH 12 (Adjust pH immediately after elution).
Only for research applications, not for diagnostic or therapeutic use!