Spot-peptide

Description
Spot-peptide for elution

Sequence
PDRVRAVSHWSS

Applications
Elution of Spot-tagged fusion proteins bound to Spot-Trap®.
Note: Optimal dilutions/ concentrations should be determined by the user

Product Size Code Price Buy
Product Spot-peptide Size 1 mg Code ep-1 Price $ 85
Buy +

Form
lyophilized powder

Size
1 mg

Purity
>96% (HPLC)

Molecular Weight
1.4 kDa

Reconstitution
Reconstitute Spot peptide in PBS to a final concentration of 2 mg/ml.

Storage instructions
Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze / thaw cycles.

How can I elute bound Spot-tagged protein from the Spot-Trap in its native state?

You can elute your Spot-fusion competitively with Spot peptide. Alternatively, you can use 10 mM NaOH pH 12 (Adjust pH immediately after elution).

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Only for research applications, not for diagnostic or therapeutic use!