Alpaca anti-RFP VHH, purified recombinant binding protein

RFP-Binding-Protein, RFP-Nanobody, and RFP-single domain antibody (sdAb), mCherry-Binding-Protein, mCherry-Nanobody, and mChery single domain antibody (sdAb),

Binds to mRFP derived from the red fluorescent protein DsRed from the sea anemone Discosoma sp. and to mRFP derivatives: mCherry, mRFPruby, mPlum, tagRFP, mKate2

Coupling of dyes, biotin, etc. via NHS Ester reaction

Product Size Code Price Buy
Product RFP VHH, recombinant binding protein Size 250 µL Code rt-250 Price $ 275
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Dissociation constant KD of 5 nM (Dissociation constant KD of 5 nM (mRFP))

For Immunoprecipitation see: Fluorescent protein specificity table
For Immunofluorescence: mCherry, mRFP, mRFPruby, mPlum

Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction

Biophysical properties
Molecular weight MW: 14.9 kDa
Molecular extinction coefficient ε: 30,035 L·Mol−1·cm−1
Isoelectrical point pI: 9.3 (theoretical)

Purified recombinant antibody fragment, monoclonal

Protein concentration
1 mg/mL

Storage buffer
25mM TAPS pH 8.5, 137 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide

How to cite this product
RRID: AB_2631365

Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.

Do not freeze.

Are the Nanobodies monoclonal or polyclonal?

The Nanobodies are recombinant/monoclonal.

What are the biophysical parameters of the Nanobodies?

Name MW (kDa) Molar Extinction Coefficient
(M-1 cm-1)
Theoretical pI
GFP VHH 13.9 27.055 9.4
GST VHH 14.8 28.545 8.2
Halo VHH 14.8 23.045 9.4
Histon VHH 14.4 25.565 10.2
MBP VHH 14.1 27.055 10.0
Mdm4/Hdmx VHH 13.6 14.565 10.1
MK2 VHH 14.4 32.555 9.7

mNeonGreen VHH

14.3 21.680 8.0
Myc VHH 13.5 23.045 9.6
p53 C-term VHH 14.8 34.170 9.2
p53 N-term VHH 13.0 20.065 9.7
PARP1 VHH 14.4 17.670 9.2
RFP VHH 14.9 30.035 9.3
SNAP/CLIP-tag VHH 13.1 24.075 9.8
Spot VHH (bivalent) 30.3 46.340 5.3
TurboGFP VHH 13.6 18.575 9.5
Vimentin VHH 26.7 48.150 10.0

Do the Nanobodies have any tags?

Yes, the Nanobodies have a C-terminal His6-tag.

Extraordinary stable & reliable binding

  • Dissociation constant KD of 5 nM
  • Applications validated and characterized

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Only for research applications, not for diagnostic or therapeutic use!