Rat monoclonal [16D10] to proliferating cell nuclear antigen (PCNA)
16D10 recognizes PCNA from human, mouse, rat, hamster. Due to sequence homology 16D10 is predicted to react with cow, dog, sheep.
Western Blot: 1:2,000
Immunoprecipitation: 25 μL/IP
|Product PCNA antibody [16D10]||Size 25 μL||Code 16d10-25||Price $ 115||Buy +|
|Product PCNA antibody [16D10]||Size 100 μL||Code 16d10-100||Price $ 315||Buy +|
Host species & clonality
20 μL; 100 μL
0.09 % Sodium azide
Shipped on blue ice. Upon receipt store at 4°C. Stable for one year.
- Rottach, A. Kremmer, E. Nowak, D. Boisguerin, P. Volkmer, R. Cardoso, M. C. Leonhardt, H. Rothbauer, U. (2008): Generation and characterization of a rat monoclonal antibody specific for PCNA. Hybridoma (Larchmt). 27: 91-8. (PubMed)
- Spada, F. Haemmer, A. Kuch, D. Rothbauer, U. Schermelleh, L. Kremmer, E. Carell, T. Langst, G. Leonhardt, H. (2007): DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells. J Cell Biol. 176: 565-71. (PubMed)
- Schermelleh, L. Haemmer, A. Spada, F. Rosing, N. Meilinger, D. Rothbauer, U. Cardoso, M. C. Leonhardt, H. (2007): Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation. Nucleic Acids Res. 35: 4301-12. (PubMed)
Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles the DNA and acts as a stationary loading platform for multiple, transiently interacting partners participating in various DNA transactions. This essential cellular component, originally characterized as a nuclear antigen of dividing cells, is evolutionary highly conserved from yeast to human. Within the eukaryotic cell, PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex network comprising cell cycle dependent changes in expression levels, dynamics, interactions, and localization of PCNA.
Only for research applications, not for diagnostic or therapeutic use!