PCNA antibody [16D10]

Description
Rat monoclonal [16D10] to proliferating cell nuclear antigen (PCNA)

Specificity
16D10 recognizes PCNA from human, mouse, rat, hamster. Due to sequence homology 16D10 is predicted to react with cow, dog, sheep.

Applications
Western Blot: 1:2,000
Immunofluorescence: 1:2,000
Immunoprecipitation: 25 μL/IP

Product Size Code Price Buy
Product PCNA antibody [16D10] Size 25 μL Code 16d10-25 Price $ 115
Buy +
Product PCNA antibody [16D10] Size 100 μL Code 16d10-100 Price $ 315
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Isotype
IgG2b

Host species & clonality
Rat monoclonal

Form
Hybridoma supernatant

Size
20 μL; 100 μL

Preservative
0.09 % Sodium azide

RRID
AB_2631401

Storage instructions
Shipped on blue ice. Upon receipt store at 4°C. Stable for one year.

  • Rottach, A. Kremmer, E. Nowak, D. Boisguerin, P. Volkmer, R. Cardoso, M. C. Leonhardt, H. Rothbauer, U. (2008): Generation and characterization of a rat monoclonal antibody specific for PCNA. Hybridoma (Larchmt). 27: 91-8. (PubMed)
  • Spada, F. Haemmer, A. Kuch, D. Rothbauer, U. Schermelleh, L. Kremmer, E. Carell, T. Langst, G. Leonhardt, H. (2007): DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells. J Cell Biol. 176: 565-71. (PubMed)
  • Schermelleh, L. Haemmer, A. Spada, F. Rosing, N. Meilinger, D. Rothbauer, U. Cardoso, M. C. Leonhardt, H. (2007): Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation. Nucleic Acids Res. 35: 4301-12. (PubMed)

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About PCNA

Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles the DNA and acts as a stationary loading platform for multiple, transiently interacting partners participating in various DNA transactions. This essential cellular component, originally characterized as a nuclear antigen of dividing cells, is evolutionary highly conserved from yeast to human. Within the eukaryotic cell, PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex network comprising cell cycle dependent changes in expression levels, dynamics, interactions, and localization of PCNA.

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Only for research applications, not for diagnostic or therapeutic use!