ChromoTek p53 N-term-Trap Agarose

p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.

Specificity

p53

Reactivity

Human

Applications

IP, CoIP

Type

Nanobody

Conjugate

Agarose

Cat no : pta

Synonyms

p53 N-term-Trap, p53 Trap, p53-Trap, Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53



Product Information

p53-N-term-Trap Agarose is an affinity reagent for IP of p53. It consists of a p53 Nanobody/ VHH coupled to agarose beads, and which binds to the N-terminus of p53.

DescriptionImmunoprecipitation of p53 with p53-N-term-Trap Agarose.

• Pull down of human p53 isoforms alpha, beta and gamma with p53-Trap-N-term

• Immunoprecipitation of the human p53 proteome with complementary p53-C-term- and p53-N-term-Traps, e.g. p53 isoforms and variants

• No heavy & light antibody chains in your downstream application

• Short incubation time (60 min at +4 °C)

ApplicationsIP, CoIP
Specificity/Targetp53 (isoforms: alpha, beta, and gamma); epitope 1-81 aa
Tested ReactivityHuman
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Type Nanobody
ClassRecombinant
HostAlpaca
Compatibility with mass spectrometryThe p53-N-term-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631378
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Cell Death Differ

The ubiquitin ligase LIN41/TRIM71 targets p53 to antagonize cell death and differentiation pathways during stem cell differentiation.

Authors - Duong Thi Thuy Nguyen
IP

Oncogene

Antagonizing CD105 enhances radiation sensitivity in prostate cancer.

Authors - Anisha Madhav
IP

PLoS Pathog

Novel modulators of p53-signaling encoded by unknown genes of emerging viruses.

Authors - Dina Alzhanova
IP

Cancers (Basel)

AML-Related NPM Mutations Drive p53 Delocalization into the Cytoplasm with Possible Impact on p53-Dependent Stress Response.

Authors - Aleš Holoubek
CoIP

J Virol

The ORF45 protein of Kaposi Sarcoma-associated Herpesvirus (KSHV) is an inhibitor of p53 signaling during viral reactivation.

Authors - Dina Alzhanova
IP

Sci Rep

Hypoxia-inducible factor prolyl-4-hydroxylase-1 is a convergent point in the reciprocal negative regulation of NF-κB and p53 signaling pathways.

Authors - Karim Ullah