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p53-N-term-Trap

1
Immunoprecipitation of tumor suppressor protein p53: p53-N-term-Trap, an anti- N-terminus of p53 VHH coupled to agarose, (left) is benchmarked against a conventional p53 antibody coupled to agarose (right). Whereas p53-N-term-Trap binds only the p53 (lane B, bound) there are also heavy and light antibody chains present in the bound fraction (lane B) of the conventional antibody resin. Input (I), non-bound (FT) and bound (B)

Description
The ChromoTek p53-N-term-Trap is an affinity bead for immunoprecipitation of p53.
It consists of an anti-p53 Nanobody/ VHH coupled to beads and that binds to the N-terminus of p53.

Specificity
p53
Species-Reactivity: Tested on human
Isoform: Alpha, beta and gamma
Epitope: 1-81 aa

Applications
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)

Formats/matrices
p53-N-term-Trap Agarose
p53-N-term VHH, recombinant binding protein

Synonyms
p53-N-term Nanobody, p53-N-term VHH, p53-N-term Binding-Protein, or p53-N-term single domain antibody fragment (sdAb)

Benefit from p53-N-term-Trap for Immunoprecipitation (IP)

  • Pull down of human p53 isoforms alpha, beta and gamma with p53-Trap-N-term
  • Immunoprecipitation of the human p53 proteome with complementary p53-C-term- and p53-N-term-Traps, e.g. p53 isoforms and variants
  • No heavy & light antibody chains in your downstream application
  • Short incubation time (60 min at +4 °C)

Format/matrices & properties

We offer the p53-N-term VHH coupled to agarose beads or uncoupled as purified protein.

Extraordinary stable & reliable binding

  • Applications validated and characterized

About p53

The tumor suppressor protein p53 plays a major role in cell cycle control, apoptosis and senescence.

Brochure

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Only for research applications, not for diagnostic or therapeutic use!