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p53-C-term-Trap

1
Pull-down of tumor suppressor protein p53: p53-C-term-Trap, an anti-p53 Nanobody coupled to agarose, (left) is benchmarked against a conventional p53 antibody coupled to agarose (right). Whereas p53-C-term-Trap binds only the p53 (lane B, bound) there are also heavy and light antibody chains present in the bound fraction (lane B) of the conventional antibody resin. Input (I), non-bound (FT) and bound (B)

Description
The ChromoTek p53-C-term-Trap is an affinity resin for immunoprecipitation of p53.
It consists of an anti-p53 Nanobody/ VHH coupled to beads that binds to the C-terminus of p53.

Specificity
p53
Species-Reactivity: Tested on human, mouse
Isoform: Alpha
Epitope: 302-393 aa

Applications
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)

Formats/matrices
p53-C-term-Trap Agarose
p53-C-term VHH, recombinant binding protein

Synonyms
p53-C-term VHH, p53-C-term Binding-Protein, p53-C-term Nanobody, p53-C-term, or single domain antibody fragment (sdAb)

Benefit from p53-C-term-Trap for Immunoprecipitation (IP)

  • Immunoprecipitation of human and mice p53 isoforms alpha with p53-C-term-Trap
  • Pull-down of the human p53 proteome with complementary p53-N-term- and p53-C-term-Traps, e.g. p53 isoforms and variants
  • No heavy & light antibody chains in your downstream application
  • Short incubation time (60 min at +4°C)
  • Strong binding even under harsh washing conditions

Format/matrices & properties

We offer the p53-C-term VHH coupled to agarose beads or uncoupled as purified protein.

Extraordinary stable & reliable binding

  • Fully applications validated and characterized

About p53

The tumor suppressor protein p53 plays a major role in cell cycle control, apoptosis and senescence.

Brochure

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Only for research applications, not for diagnostic or therapeutic use!