Nanobodies/ VHHs

ChromoTek’s unconjugated Nanobodies or VHHs are well characterized and validated for the listed applications. Because the molecular weight of these single-domain antibodies (sdAb) is just about 15 kDa, VHHs are only one tenth the size of a conventional IgG antibody (150 kDa) or less than a third the size of a Fab fragment (50 kDa). In combination with their high chemical and thermal stability plus high affinity, these binding proteins are ideal for multiple types of affinity assays in biochemistry and microscopy, including immunoprecipitation, immunofluorescence, super resolution microscopy, surface plasmon resonance (SPR), biolayer interferometry (BLI), switchSENSE technology, etc.

GFP VHH

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Code Description Size Price Qty
Code gt-250 Description GFP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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GFP VHH, biotinylated

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Code Description Size Price Qty
Code gtb-250 Description GFP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 500
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GST VHH

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Code Description Size Price Qty
Code st-250 Description GST-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

Halo VHH

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Code Description Size Price Qty
Code ot-250 Description Halo-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

Histone VHH

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Code Description Size Price Qty
Code tt-250 Description Histone Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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MBP VHH

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Code Description Size Price Qty
Code mbt-250 Description MBP-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

MK2 VHH

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Code Description Size Price Qty
Code mt-250 Description MK2-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

mNeonGreen VHH

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Code Description Size Price Qty
Code nt-250 Description mNeonGreen-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

Myc VHH

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Code Description Size Price Qty
Code yt-250 Description Myc-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

p53 C-term VHH

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Code Description Size Price Qty
Code pt2-250 Description p53 C-term-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

p53 N-term VHH

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Code Description Size Price Qty
Code pt-250 Description p53 N-term-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

PARP1 VHH

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Code Description Size Price Qty
Code xt-250 Description PARP1-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

RFP VHH

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Code Description Size Price Qty
Code rt-250 Description RFP-Binding-Protein, 1 mg / mL Size 250 µL Price $ 275
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SNAP/CLIP-tag® VHH

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Code Description Size Price Qty
Code wt-250 Description SNAP/CLIP-tag®-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

Spot VHH

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Code Description Size Price Qty
Code etb-250 Description Spot-Binding-Protein, 1 mg / mL Size 250 µL Price $ 455
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TurboGFP VHH

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Code Description Size Price Qty
Code tbt-250 Description TurboGFP-Binding-Protein, 1 mg / mL Size 250 µL Price Please inquire

Vimentin VHH

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Code Description Size Price Qty
Code vt-250 Description Vimentin Binding-Protein, 1 mg / mL Size 250 µL Price $ 500
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Ligand capture and Affinity Determination Methods

Label-free methods for the measurement and characterization of biomolecular interactions are very popular and widely used, e.g. when conducting binding assays and screenings. Here, on-rates, off-rates, and affinities are determined based on an experimental set-up, in which a ligand is captured to a sensor’s surface; the ligand then interacts with an analyte that the sensor is probed with. Applied assay technologies comprise surface plasmon resonance (SPR), e.g. from BiacoreTM/Cytiva (formerly GE Healthcare), bio-layer interferometry (BLI), e.g. from FortéBio/Sartorius, and switchSENSE® from Dynamic Biosensors. The stable, uniform and oriented, i.e. site-directed, immobilization of the ligand is of considerable importance for the reliable determination of the binding kinetics of ligand to analyte. 
In general, these assays are distinguished by the immobilization of the ligand to the sensor’s surface: 

  1. Direct immobilization assays: 
    Covalent linking via lysines or cysteines of the ligand and click chemistry 
  2. Indirect immobilization assays or capture kinetics assays:
    Capturing the ligand via specific binding molecules like antibodies or Nanobodies in a site-directed, i.e. Fc-domain specific manner or via antibodies or Nanobodies against protein- or peptide-tags like GFP, GST, MBP, Spot-tag, His-tag, etc.

Common capture molecules for antibodies are anti-Fc specific antibodies and Nanobodies like Nano-CaptureLigands™, or protein A, protein G, protein A/G. 
Compared to direct immobilization assays, the benefits of capture kinetics assays are 

  1. Site-directed immobilization, 
  2. Regeneration is a standard procedure and does not depend on the captured molecule, because the ligand is removed after every cycle, 
  3. The ability to capture unpurified monoclonal antibodies from crude samples, 
  4. Easier optimization of capture ligands’ density.
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Only for research applications, not for diagnostic or therapeutic use!