Nano-CaptureLigands
6
Description
Nano-CaptureLigands™ for the site-directed and gentle immobilization of antibodies in biosensor and ELISA assays. Nano-CaptureLigands specifically capture non-biotinylated immunoglobulins to streptavidin/avidin. Nano-CaptureLigands are biotinylated VHHs/ Nanobodies.
Advanced immobilization of antibodies
Site-directed and gentle immobilization of antibodies
No antibody biotinylation required
Selective immobilization from crude samples
Stable baseline with negligible antibody dissociation
Frequent regeneration for multiple reuse
Applications
Antibody capture to biosensors used in biolayer interferometry (BLI) and surface plasmon resonance (SPR), and to microplates used in enzyme-linked immunosorbent assays (ELISA)
Nano-CaptureLigands for the immobilization of
Human Ig lambda
Human IgE
Human IgG
Mouse IgE
Mouse IgG1
Mouse IgG2a
Mouse IgG2b
Rabbit IgG
Nano-CaptureLigands specificity overview
Nano-CaptureLigand human Ig, lambda
| Code | Description | Size | Price | Qty |
|---|---|---|---|---|
| Code shuLB-1-10 | Description Nano-CaptureLigand™ human Ig, lambda-LC-specific VHH, biotinylated | Size 10 µL | Price $ 130 | |
| Code shuLB-1-100 | Description Nano-CaptureLigand™ human Ig, lambda-LC-specific VHH, biotinylated | Size 100 µL | Price $ 480 |
Nano-CaptureLigand human IgE
| Code | Description | Size | Price | Qty |
|---|---|---|---|---|
| Code shuEB-1-10 | Description Alpaca anti-human IgE, recombinant VHH, Biotin | Size 10 µL | Price $ 130 | |
| Code shuEB-1-100 | Description Alpaca anti-human IgE, recombinant VHH, Biotin | Size 100 µL | Price $ 480 |
Nano-CaptureLigand human IgG
| Code | Description | Size | Price | Qty |
|---|---|---|---|---|
| Code shurbGB-1-10 | Description Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated | Size 10 µL | Price $ 130 | |
| Code shurbGB-1-100 | Description Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated | Size 100 µL | Price $ 480 |
Nano-CaptureLigand mouse IgE
| Code | Description | Size | Price | Qty |
|---|---|---|---|---|
| Code smsEB-1-10 | Description Alpaca anti-mouse IgE, recombinant VHH, Biotin | Size 10 µL | Price $ 130 | |
| Code smsEB-1-100 | Description Alpaca anti-mouse IgE, recombinant VHH, Biotin | Size 100 µL | Price $ 480 |
Nano-CaptureLigand mouse IgG1
| Code | Description | Size | Price | Qty |
|---|---|---|---|---|
| Code smsG1B-1-10 | Description Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Biotin | Size 10 µL | Price $ 130 | |
| Code smsG1B-1-100 | Description Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Biotin | Size 100 µL | Price $ 480 |
Nano-CaptureLigand mouse IgG2a
| Code | Description | Size | Price | Qty |
|---|---|---|---|---|
| Code smsG2aB-1-10 | Description Alpaca anti-mouse IgG2a, Fc-specific recombinant VHH, Biotin | Size 10 µL | Price $ 130 | |
| Code smsG2aB-1-100 | Description Alpaca anti-mouse IgG2a, Fc-specific recombinant VHH, Biotin | Size 100 µL | Price $ 480 |
Nano-CaptureLigand mouse IgG2b
| Code | Description | Size | Price | Qty |
|---|---|---|---|---|
| Code smsG2bB-1-10 | Description Alpaca anti-mouse IgG2b, Fc-specific recombinant VHH, Biotin | Size 10 µL | Price $ 130 | |
| Code smsG2bB-1-100 | Description Alpaca anti-mouse IgG2b, Fc-specific recombinant VHH, Biotin | Size 100 µL | Price $ 480 |
Nano-CaptureLigand rabbit IgG
| Code | Description | Size | Price | Qty |
|---|---|---|---|---|
| Code shurbGB-1-10 | Description Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated | Size 10 µL | Price $ 130 | |
| Code shurbGB-1-100 | Description Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated | Size 100 µL | Price $ 480 |
Applications
- Characterization of antibodies’ binding kinetics and affinity
- Antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma
- Detection of antibodies and antigens
- Epitope binning
The next level of antibody immobilization to biosensors
ChromoTek’s Nano-CaptureLigands immobilize non-biotinylated antibodies to streptavidin/avidin coated biosensors used in surface plasmon resonance (SPR) and bio-layer interferometry (BLI) assays like the FortéBio Octet® systems.
This immobilization is very strong because of the high affinity of the Nano-CaptureLigands. As a result, there is a stable baseline without significant dissociation of captured antibodies.
Nano-CaptureLigands enable the advanced characterization of antibodies and antibody fragments including detection and quantitation plus binding kinetics and affinity.
Nano-CaptureLigands can be regenerated >10 times for multiple reuse.
Their high specificity enables antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples.
The next level of antibody immobilization for ELISA
ChromoTek’s Nano-CaptureLigands enable the site-directed immobilization of capture antibodies for sandwich ELISAs. They combine the site-specificity of protein A, G, and L with the stability of the biotin/streptavidin interaction. Because of the Nano-CaptureLigands’ subclass specificity, a broader range of capture and detection antibody pairs can be used in ELISA.
The Nano-CaptureLigands’ high affinity results in a strong immobilization of antibodies even at low antibody concentrations.
Nano-CaptureLigands are thoroughly validated
- Characterized and validated: DNA and protein sequences, affinity, melting temperature
- Recombinantly produced, virtually without lot-to-lot variation
- Manufacturing process is totally animal free, though Nanobodies are generated from immunization of alpacas and llamas
Only for research applications, not for diagnostic or therapeutic use!