Nano-CaptureLigands

Kinetics of the binding of a rabbit IgG antibody to its target in BLI. α-rabbit IgG captured to a streptavidin biosensor tip with Nano-CaptureLigand rabbit IgG.

Nano-CaptureLigand immobilizing an antibody in a site-directed manner

Nano-CaptureLigands are derived from Alpaca heavy chain antibodies. These are biotinylated Nanobodies/VHHs against immunoglobulins.

ELISA capture of an antibody using Nano-CaptureLigands.1: Nano-CaptureLigand coating. 2: Immobilization of capture antibody. 3: Antigen binding. 4: Binding or primary and detection antibody.

Left: Site-directed and gentle immobilization of antibodies using Nano-CaptureLigands. No antibody biotinylation is required. Note the homogeneous orientation of all captured antibodies. Right: Direct antibody biotinylation results in random, non-directed immobilization of the antibodies. The random biotinylation may alter the antibody’s paratope or immobilize the antibody in an orientation that compromises its binding to the target. Note that only a fraction of the immobilized antibodies is capable of binding their targets.

Description
Nano-CaptureLigands™ for the site-directed and gentle immobilization of antibodies in biosensor and ELISA assays. Nano-CaptureLigands specifically capture non-biotinylated immunoglobulins to streptavidin/avidin. Nano-CaptureLigands are biotinylated V_HHs/ Nanobodies.

Advanced immobilization of antibodies
Site-directed and gentle immobilization of antibodies
No antibody biotinylation required
Selective immobilization from crude samples
Stable baseline with negligible antibody dissociation
Frequent regeneration for multiple reuse

Applications
Antibody capture to biosensors used in biolayer interferometry (BLI) and surface plasmon resonance (SPR), and to microplates used in enzyme-linked immunosorbent assays (ELISA)

Nano-CaptureLigands for the immobilization of
Human Ig lambda
Human IgG
Mouse IgG1
Mouse IgG2a
Mouse IgG2b
Rabbit IgG

Nano-CaptureLigands specificity overview

Product Listing
Applications

Nano-CaptureLigand human Ig, lambda

PRICE Nano-CaptureLigand human Ig, lambda

ELISA SPR/ BLI

Code	Description	Size	Price	Qty
Code shuLB-1-10	Description Nano-CaptureLigand™ human Ig, lambda-LC-specific VHH, biotinylated	Size 10 µL	Price $ 130	Qty + +
Code shuLB-1-100	Description Nano-CaptureLigand™ human Ig, lambda-LC-specific VHH, biotinylated	Size 100 µL	Price $ 480	Qty + +

Nano-CaptureLigand human IgG

PRICE Nano-CaptureLigand human IgG

ELISA SPR/ BLI

Code	Description	Size	Price	Qty
Code shurbGB-1-10	Description Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated	Size 10 µL	Price $ 130	Qty + +
Code shurbGB-1-100	Description Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated	Size 100 µL	Price $ 480	Qty + +

Nano-CaptureLigand mouse IgG1

PRICE Nano-CaptureLigand mouse IgG1

ELISA SPR/ BLI

Code	Description	Size	Price	Qty
Code smsG1B-1-10	Description Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Biotin	Size 10 µL	Price $ 130	Qty + +
Code smsG1B-1-100	Description Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Biotin	Size 100 µL	Price $ 480	Qty + +

Nano-CaptureLigand mouse IgG2a

PRICE Nano-CaptureLigand mouse IgG2a

ELISA SPR/ BLI

Code	Description	Size	Price	Qty
Code smsG2aB-1-10	Description Alpaca anti-mouse IgG2a, Fc-specific recombinant VHH, Biotin	Size 10 µL	Price $ 130	Qty + +
Code smsG2aB-1-100	Description Alpaca anti-mouse IgG2a, Fc-specific recombinant VHH, Biotin	Size 100 µL	Price $ 480	Qty + +

Nano-CaptureLigand mouse IgG2b

PRICE Nano-CaptureLigand mouse IgG2b

ELISA SPR/ BLI

Code	Description	Size	Price	Qty
Code smsG2bB-1-10	Description Nano-CaptureLigand™ mouse IgG2b, Fc-specific VHH, biotinylated	Size 10 µL	Price $ 130	Qty + +
Code smsG2bB-1-100	Description Nano-CaptureLigand™ mouse IgG2b, Fc-specific VHH, biotinylated	Size 100 µL	Price $ 480	Qty + +

Nano-CaptureLigand rabbit IgG

PRICE Nano-CaptureLigand rabbit IgG

ELISA SPR/ BLI

Code	Description	Size	Price	Qty
Code shurbGB-1-10	Description Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated	Size 10 µL	Price $ 130	Qty + +
Code shurbGB-1-100	Description Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated	Size 100 µL	Price $ 480	Qty + +

Applications

Characterization of antibodies’ binding kinetics and affinity
Antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma
Detection of antibodies and antigens
Epitope binning

The next level of antibody immobilization to biosensors

ChromoTek’s Nano-CaptureLigands immobilize non-biotinylated antibodies to streptavidin/avidin coated biosensors used in surface plasmon resonance (SPR) and bio-layer interferometry (BLI) assays like the FortéBio Octet^® systems.
This immobilization is very strong because of the high affinity of the Nano-CaptureLigands. As a result, there is a stable baseline without significant dissociation of captured antibodies.
Nano-CaptureLigands enable the advanced characterization of antibodies and antibody fragments including detection and quantitation plus binding kinetics and affinity.
Nano-CaptureLigands can be regenerated >10 times for multiple reuse.
Their high specificity enables antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples.

Comparison of BLI/SPR immobilization methods

The next level of antibody immobilization for ELISA

ChromoTek’s Nano-CaptureLigands enable the site-directed immobilization of capture antibodies for sandwich ELISAs. They combine the site-specificity of protein A, G, and L with the stability of the biotin/streptavidin interaction. Because of the Nano-CaptureLigands’ subclass specificity, a broader range of capture and detection antibody pairs can be used in ELISA.
The Nano-CaptureLigands’ high affinity results in a strong immobilization of antibodies even at low antibody concentrations.

Comparison of ELISA immobilization methods

Nano-CaptureLigands are thoroughly validated

Characterized and validated: DNA and protein sequences, affinity, melting temperature
Recombinantly produced, virtually without lot-to-lot variation
Manufacturing process is totally animal free, though Nanobodies are generated from immunization of alpacas and llamas

Only for research applications, not for diagnostic or therapeutic use!