Nano-CaptureLigand human IgG

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Description
The Nano-CaptureLigand™ human IgG captures and immobilizes the Fc-fragment of human IgG antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human IgG antibodies with high affinity and in a site-directed manner.
Specificity: Fc-fragment of human IgG1, IgG2, IgG3, and IgG4; Co-reactive to rabbit IgG
Nano-CaptureLigands specificity overview
Conjugates: Biotin
Applications
Human IgG capture to biosensors used in BLI and SPR
Human IgG immobilization for ELISA
Advanced immobilization of antibodies
Specific, stable, and site-directed immobilization of non-biotinylated human IgG antibodies to streptavidin/avidin
Product | Size | Code | Price | Buy |
---|---|---|---|---|
Product Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated | Size 10 µL | Code shurbGB-1-10 | Price $ 130 |
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Product Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated | Size 100 µL | Code shurbGB-1-100 | Price $ 480 |
Buy
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Product Type
Capture Nanobody (VHH)
Applications
Immobilization of human antibodies on avidin and streptavidin surfaces for Bio-Layer Interferometry (BLI), Surface Plasmon Resonance (SPR), and ELISA
Target/ Specificity
Fc-fragment of human IgG1, IgG2, IgG3, and IgG4
Co-reactive to Fc-fragment of rabbit IgG
Cross-reactivity
No cross-reactivity to human IgA, IgE, IgM; mouse IgG1, IgG2a, Ig2b, IG2c, IgG3; rat IgG1, IgG2a, IgG2b, IgG2c; sera from goat, guinea pig, rat, sheep
Cross-reactivity to macaque (cynomolgus monkey) serum
Affinity (Kd) of monovalent (1:1) binding mode
Human IgG1: 1.4 nM; Human IgG2: 0.4 nM; Human IgG3: 9.0 nM; Human IgG4: 0.2 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).
Concentration
1 g/L (71 μM)
Conjugate
Biotin
Degree of biotinylation
On average 1-2 biotin molecules per Nanobody
Format
Alpaca single domain antibody, monovalent
Host
Alpaca-derived, recombinantly produced in bacteria
Clonality
Monoclonal
Clone
CTK0101 (VHH0238)
How to cite this product
Nano-CaptureLigand™ human IgG/ rabbit IgG, Fc-specific VHH, biotinylated
RRID: AB_2848185
Synonyms
Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody
Validation
Application validated for ELISA and BLI (FortéBio Octet® systems)
Determination of cross-reactivity, subclass specificity, sequence, affinity, and melting temperature
Purity
Recombinantly expressed and purified via His-tag
Form
Buffered aqueous solution
Storage buffer
25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide
Storage conditions
Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F
Stability
Stable for 1 year at +4°C/+40°F
Shipment
Shipped at ambient temperature
Protocol
Protocol Nano-CaptureLigand human IgG/ rabbit IgG, Fc-specific VHH, biotinylated (PDF)
SDS
SDS Nano-CaptureLigand™ human IgG/rabbit IgG, Fc-specific VHH, biotinylated (PDF)
Nano-CaptureLigand Specificity
Nano-CaptureLigands specificity overview (PDF)
Application note
Nano-CaptureLigands for COVID-19 antibodies capture in BLI binding kinetics assay (PDF)
Comparison of immobilization methods
Applications
- Detection and quantitation of human IgG antibodies and their antigens
- Characterization of human IgG antibodies’ binding kinetics and affinity
- Human IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma
- Epitope binning
The next level of antibody immobilization to BLI and SPR biosensors
- Site-directed and specific immobilization of the Fc-fragment of human IgG to streptavidin coated biosensors without biotinylation of the antibody
- Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)
- High affinity for stable baseline without significant dissociation
- >10x regeneration for multiple reuse
- High specificity enables human IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples
The next level of antibody immobilization for ELISA
- Compatible to enzyme-linked immunosorbent assays (ELISA)
- Site-directed immobilization of Fc-fragment of human IgG antibodies to streptavidin/avidin coated surfaces of sandwich ELISAs without biotinylation of antibody
- Combine the site-specificity of protein A, G, and L with the stability of the biotin/streptavidin interaction
- Human IgG specificity allows the application of a broad range of detection antibodies
- High affinity for strong immobilization of human IgG antibodies even at low antibody concentrations
Nano-CaptureLigands are comprehensively validated
- Characterized: DNA and protein sequences, affinity, melting temperature
- Recombinantly produced, virtually without lot-to-lot variation
- Manufacturing process is animal free
Only for research applications, not for diagnostic or therapeutic use!