Nano-CaptureLigand human IgE

Description
The ChromoTek Nano-CaptureLigand™ human IgE, VHH, biotinylated is used for the site-directed and specific immobilization of human IgE in biosensor and ELISA assays. It captures non-biotinylated human IgE antibodies to streptavidin/avidin. Nano-CaptureLigand human IgE, VHH, biotinylated comprises a monoclonal biotinylated VHH/ Nanobody.

Specificity: Human IgE antibodies
Does not bind to human IgG1 (kappa and lambda LC), IgG2, IgG3, IgG4, IgA, IgM;
mouse IgE, Ig1 (kappa and lambda LC), IgG2a, IgG2b, IgG2c, IgG3; rabbit IgG
Nano-CaptureLigands specificity overview

Conjugates: Biotin

Applications
Human IgE capture to biosensors used in BLI and SPR
Human IgE immobilization for ELISA

Advanced immobilization of antibodies
Specific, stable, and site-directed immobilization of non-biotinylated human IgE antibodies to streptavidin/avidin

Product Size Code Price Buy
Product Nano-CaptureLigand™ human IgE, VHH, biotinylated Size 10 µL Code shuEB-1-10 Price $ 130
Buy +
Product Nano-CaptureLigand™ human IgE, VHH, biotinylated Size 100 µL Code shuEB-1-100 Price $ 480
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Product Type
Capture Nanobody (VHH)

Applications
Immobilization of human IgE antibodies to avidin and streptavidin surfaces for Bio-Layer Interferometry (BLI), Surface Plasmon Resonance (SPR), and ELISA

Target/ Specificity 
Human IgE

Cross-reactivity
No cross-reactivity to human IgG1 (kappa and lambda LC), IgG2, IgG3, IgG4, IgA, IgM; mouse IgE, Ig1 (kappa and lambda LC), IgG2a, IgG2b, IgG2c, IgG3; rabbit IgG

Affinity (Kd) of monovalent (1:1) binding mode
0.72 nM
Apparent affinity may be higher for full IgEs due to avidity effects (1 antibody captured by 2 Nanobodies).

Concentration 
1 g/L (68 μM)

Conjugate
Biotin

Degree of biotinylation 
On average 1-2 biotin molecules per Nanobody

Format 
Alpaca single domain antibody, monovalent

Host
Alpaca-derived, recombinantly produced in bacteria

Clonality
Monoclonal

Clone
CTK0111 (VHH0428)

How to cite this product
Nano-CaptureLigand™ human IgE, VHH, biotinylated
RRID: AB_2864256

Synonyms 
Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Validation 
Application validated for ELISA and BLI (FortéBio Octet® systems)
Determination of cross-reactivity, subclass specificity, sequence, affinity, and melting temperature

Purity
Recombinantly expressed and purified via His-tag

Form
Buffered aqueous solution

Storage buffer 
25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide

Storage conditions 
Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F

Stability
Stable for 1 year at +4°C/+40°F

Shipment 
Shipped at ambient temperature

Applications

  • Detection and quantitation of human IgE antibodies and their antigens
  • Characterization of human IgE antibodies’ binding kinetics and affinity
  • Human IgE antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma
  • Epitope binning

The next level of antibody immobilization to BLI and SPR biosensors

  • Gentle, site-directed immobilization of human IgE antibodies to streptavidin coated biosensors without biotinylation of the antibody
  • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)
  • High affinity for stable baseline without significant dissociation
  • >10x regeneration for multiple reuse
  • High specificity enables human IgE antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

Comparison of BLI/SPR immobilization methods

The next level of antibody immobilization for ELISA

  • Compatible to enzyme-linked immunosorbent assays (ELISA) 
  • Site-directed immobilization of human IgE capture antibodies to streptavidin/avidin coated surfaces of sandwich ELISAs without biotinylation of antibody
  • Combine the site-specificity of protein A, G, and L with the stability of the biotin/streptavidin interaction
  • Human IgE subclass specificity allows the application of a broad range of detection antibodies 
  • High affinity for strong immobilization of human IgE antibodies even at low antibody concentrations

Comparison of ELISA immobilization methods

Nano-CaptureLigands are comprehensively validated

  • Characterized: DNA and protein sequences, affinity, melting temperature
  • Recombinantly produced, virtually without lot-to-lot variation
  • Manufacturing process is animal free

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Only for research applications, not for diagnostic or therapeutic use!