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Nano-CaptureLigand human Ig, lambda

4
BLI binding kinetics of a human anti-GFP Fab fragment to eGFP. A synthetic human anti-GFP lambda Fab fragment was immobilized using Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated on FortéBio Streptavidin (SA) Biosensors and assayed with different concentrations of eGFP (ChromoTek EGFP, recombinant protein).
Immobilization of the Nano-CaptureLigand (green) on Biosensor (white) for biolayer interferometry (BLI) followed by capture of lambda light chain of human Ig antibody (orange). The setup is ready for characterization of human IgG antibodies’ binding kinetics and affinity to substrate (red), human IgG antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma.
ELISA capture of a human IgG1 lambda antibody using Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated. 50 nM Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated was used for coating on an avidin-coated MaxiSorp plate. Human IgG1 lambda antibody was titrated in a 1:2 dilution series and detected with an alkaline phosphatase-conjugated detection antibody.

Description
The Nano-CaptureLigand™ human Ig, lambda-LC captures and immobilizes human Ig lambda light chain (LC) antibodies to avidin/streptavidin coated surfaces used in BLI, SPR, and ELISA assays. It consists of a biotinylated Nanobody that specifically binds to human Ig lambda LC antibodies with high affinity and in a site-directed manner.

Specificity: Lambda light chain of human Ig lambda antibodies
Nano-CaptureLigands specificity overview

Conjugates: Biotin

Applications
Human Ig lambda capture to biosensors used in BLI and SPR
Human Ig lambda immobilization for ELISA

Advanced immobilization of antibodies
Specific, stable, and site-directed immobilization of non-biotinylated human Ig lambda antibodies to streptavidin/avidin

 

Product Size Code Price Buy
Product Nano-CaptureLigand™ human Ig, lambda-LC-specific VHH, biotinylated Size 10 µL Code shuLB-1-10 Price $ 130
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Product Nano-CaptureLigand™ human Ig, lambda-LC-specific VHH, biotinylated Size 100 µL Code shuLB-1-100 Price $ 480
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Product Type
Capture Nanobody (VHH)

Applications
Immobilization of human Ig lambda-LC antibodies on avidin and streptavidin surfaces for Bio-Layer Interferometry (BLI), Surface Plasmon Resonance (SPR), and ELISA

Target/ Specificity 
Lambda-LC of human Ig lambda antibodies

Cross-reactivity
No cross-reactivity to human Ig kappa; goat IgG; mouse IgG; rabbit IgG Fc; rat IgG
Cross-reactivity to rabbit IgG Fab

Affinity (Kd) of monovalent (1:1) binding mode
0.1 nM
Apparent affinity may be higher for full IgGs due to avidity effects (1 antibody captured by 2 Nanobodies).

Concentration 
1 g/L (68 μM)

Conjugate
Biotin

Degree of biotinylation 
On average 1-2 biotin molecules per Nanobody

Format 
Alpaca single domain antibody, monovalent

Host
Alpaca-derived, recombinantly produced in bacteria

Clonality
Monoclonal

Clone
CTK0110 (VHH0369)

How to cite this product
Nano-CaptureLigand™ human Ig, lambda-LC-specific VHH, biotinylated
RRID: AB_2848190

Synonyms 
Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

Validation 
Application validated for ELISA and BLI (FortéBio Octet® systems)
Determination of cross-reactivity, subclass specificity, sequence, affinity, and melting temperature

Purity
Recombinantly expressed and purified via His-tag

Form
Buffered aqueous solution

Storage buffer 
25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % sodium azide

Storage conditions 
Upon receipt store at +4°C/+40°F. Optional: Aliquot upon arrival and store at -20°C/-4°F

Stability
Stable for 1 year at +4°C/+40°F

Shipment 
Shipped at ambient temperature

Applications

  • Detection and quantitation of human Ig lambda light chain antibodies and their antigens
  • Characterization of human Ig lambda antibodies’ binding kinetics and affinity
  • Human Ig lambda antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma
  • Epitope binning

The next level of antibody immobilization to BLI and SPR biosensors

  • Site-directed and specific immobilization of the Fab-fragment of human Ig through the lambda light chain to streptavidin coated biosensors without biotinylation of the antibody
  • Compatible to surface plasmon resonance (SPR) and biolayer interferometry (BLI) assays (e.g. FortéBio Octet® systems)
  • High affinity for stable baseline without significant dissociation
  • >10x regeneration for multiple reuse
  • High specificity enables human Ig lambda antibody discovery and screening in crude liquids like hybridoma supernatant, serum, and plasma samples

 

Comparison of BLI/SPR immobilization methods

The next level of antibody immobilization for ELISA

  • Compatible to enzyme-linked immunosorbent assays (ELISA) 
  • Site-directed immobilization of human Ig lambda light chain antibodies to streptavidin/avidin coated surfaces of sandwich ELISAs without biotinylation of antibody
  • Combine the site-specificity of protein A, G, and L with the stability of the biotin/streptavidin interaction
  • Human Ig lambda subclass specificity allows the application of a broad range of detection antibodies 
  • High affinity for strong immobilization of human Ig lambda antibodies even at low antibody concentrations

 

Comparison of ELISA immobilization methods

Nano-CaptureLigands are comprehensively validated

  • Characterized: DNA and protein sequences, affinity, melting temperature
  • Recombinantly produced, virtually without lot-to-lot variation
  • Manufacturing process is animal free

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Only for research applications, not for diagnostic or therapeutic use!