Myc-Trap

Description
The ChromoTek Myc-Trap® is an affinity resin for immunoprecipitation and purification of Myc-tagged proteins.

It comprises an anti-Myc-tag Nanobody/ VHH coupled to beads.

Specificity
The Myc-Trap recognizes the Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. The Myc-Trap does not bind endogenous c-myc.

Applications
Immunoprecipitation (IP) / Co-IP

Affinity purification
Mass spectrometry (MS)
On-bead enzyme assays
ChIP/ RIP analysis

Formats/matrices
Myc-Trap Agarose
Myc-Trap Magnetic Agarose
Myc VHH, recombinant binding protein

Synonyms
Myc Nanobody, anti-Myc VHH, Myc-binding protein or Myc single domain antibody fragment (sdAb)

Benefit from Myc-Trap for Immunoprecipitation (IP)

  • Efficient immunoprecipitation of Myc-tag fusion proteins, low background
  • No heavy & light antibody chains in downstream applications
  • Easy elution of native proteins
  • Harsh washing conditions

Format/matrices & properties

We offer the anti-Myc VHH coupled to agarose beads and magnetic agarose beads, or uncoupled as purified protein.

  Myc-Trap Agarose Myc-Trap Magnetic Agarose
matrix agarose magnetic agarose
color white black
particle size 90 µm 40 µm
magnetic no yes
may be centrifuged up to 2,500 x g 800 x g

Extraordinary stable & reliable binding

  • Dissociation constant KD of 500 nM for 1x Myc
  • Dissociation constant KD of 0.5 nM for 2x Myc
  • Compatible to harsh wash conditions such as  2 M NaCl, 2 % Nonidet P40 Substitute
  • Applications validated and characterized

About Myc-tag

The Myc-tag is derived from the human c-myc oncogene. It is a commonly used epitope tag to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems. Myc-tag is also named c-myc tag.

The sequence motif is EQKLISEEDL.

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Only for research applications, not for diagnostic or therapeutic use!