Myc-tag antibody [9E1]

2
Description
Rat monoclonal [9E1] to Myc-tag
Specificity
The antibody recognizes the Myc-tag sequence EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein.
Applications
Western Blot: 1:1,000
Immunofluorescence: 1:500
ELISA: 1:1,000
Immunoprecipitation: 5 µg/ IP
Product | Size | Code | Price | Buy |
---|---|---|---|---|
Product Myc-tag antibody [9E1] | Size 20 µL | Code 9e1-20 | Price $ 100 |
Buy
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Product Myc-tag antibody [9E1] | Size 100 µL | Code 9e1-100 | Price $ 245 |
Buy
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Isotype
IgG1
Host species & clonality
Rat monoclonal
Form
Purified antibody
Size
20 μL; 100 μL
Purity
Protein G purified
Protein concentration
1.0 mg/mL
Storage buffer
PBS, preservative: 0.09 % Sodium azide
RRID
AB_2631398
Storage instructions
Shipped on blue ice. Upon receipt store at 4°C. Stable for one year.
Do not freeze
Does the Myc-tag antibody [9E1] bind endogenous c-Myc protein?
We could not detect binding of the Myc-tag antibody [9E1] to endogenous c-Myc protein. Some epitope residues that have shown to be crucial for binding to the Myc-tag antibody are buried in the three-dimensional structure of the c-Myc protein. Hence, under native conditions, c-Myc protein is not a suitable binding partner for the Myc-tag antibody [9E1].
Can I use the Myc-tag antibody [9E1] for immunoprecipitation?
Yes, you can use the Myc-tag antibody [9E1] for immunoprecipitation and immobilize it on beads through protein A or G.
Only for research applications, not for diagnostic or therapeutic use!