Myc-peptide (2x)

Description
2x Myc-peptide, 2x c-Myc peptide

Sequence
EQKLISEEDLEQKLISEEDL
The single myc-tag motif EQKLISEEDL corresponds to amino acids 410-19 of the C-terminal domain of human c-myc.

Applications
Elution of myc-tagged fusion proteins bound to Myc-Trap® or anti-Myc antibodies.

Note: Optimal dilutions/concentrations should be determined by the user

Product Size Code Price Buy
Product 2x Myc-peptide Size 1 mg Code 2yp-1 Price $ 105
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Form
lyophilized powder

Size
1 mg

Purity
>96% (HPLC)

Molecular Weight
2.4 kDa

Reconstitution
Reconstitute 2xMyc-peptide in phosphate buffered saline (PBS) pH 7.4 to a final concentration of 1 mg/ml.

Storage instructions
Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze/ thaw cycles.

How can I gently elute native Myc-tagged protein from the Myc-Trap?

You can elute your Myc-fusion competitively with 1x or 2x Myc-peptide. Alternatively, you can use 8 M Urea or 0.2 M glycine pH 2.5 at room temperature.

Should I use 1x myc or 2x myc peptide for elution of the Myc-Trap?

It is likely that the Myc-Trap binds several motifs of a double Myc-tag. Hence, elution of a myc-tagged fusion protein is more efficient with the 2x myc peptide. In addition, the term "myc-tag" can refer to a single or double myc-tag. To be on the safe side, we generally recommend elution with the 2x Myc peptide.

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Only for research applications, not for diagnostic or therapeutic use!