2x Myc-peptide, 2x c-Myc peptide
The single myc-tag motif EQKLISEEDL corresponds to amino acids 410-19 of the C-terminal domain of human c-myc.
Elution of myc-tagged fusion proteins bound to Myc-Trap® or anti-Myc antibodies.
Note: Optimal dilutions/concentrations should be determined by the user
|Product 2x Myc-peptide||Size 1 mg||Code 2yp-1||Price $ 105||Buy +|
Reconstitute 2xMyc-peptide in phosphate buffered saline (PBS) pH 7.4 to a final concentration of 1 mg/ml.
Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze/ thaw cycles.
How can I gently elute native Myc-tagged protein from the Myc-Trap?
You can elute your Myc-fusion competitively with 1x or 2x Myc-peptide. Alternatively, you can use 8 M Urea or 0.2 M glycine pH 2.5 at room temperature.
Should I use 1x myc or 2x myc peptide for elution of the Myc-Trap?
It is likely that the Myc-Trap binds several motifs of a double Myc-tag. Hence, elution of a myc-tagged fusion protein is more efficient with the 2x myc peptide. In addition, the term "myc-tag" can refer to a single or double myc-tag. To be on the safe side, we generally recommend elution with the 2x Myc peptide.
Only for research applications, not for diagnostic or therapeutic use!