Myc-peptide (1x)
1
Description
Myc-peptide, c-Myc-peptide
Sequence
EQKLISEEDL
corresponds to amino acids 410-419 of the C-terminus of human c-Myc
Applications
Elution of Myc-tagged fusion proteins bound to Myc-Trap® or anti-Myc antibodies, will not elute 3x Myc-tagged proteins.
Note: Optimal dilutions/concentrations should be determined by the user
| Product | Size | Code | Price | Buy |
|---|---|---|---|---|
| Product Myc-peptide (1x) | Size 1 mg | Code yp-1 | Price $ 85 |
Buy
+
|
| Product Myc-peptide (1x) | Size 10 mg | Code yp-10 | Price $ 640 |
Buy
+
|
Form
lyophilized powder
Size
1 mg; 10 mg
Purity
>96% (HPLC)
Molecular Weight
1.2 kDa
Reconstitution
Reconstitute Myc-peptide in water to a final concentration of 2 mg/ml.
Storage instructions
Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze/ thaw cycles.
How can I gently elute native Myc-tagged protein from the Myc-Trap?
You can elute your Myc-fusion competitively with 1x or 2x Myc peptide. Alternatively, you can use 8 M Urea or 0.2 M glycine pH 2.5 at room temperature.
Should I use 1x Myc or 2x Myc-peptide for elution of the Myc-Trap?
It is likely that the Myc-Trap binds several motifs of a double Myc-tag. Hence, elution of a myc-tagged fusion protein is more efficient with the 2x Myc-peptide. In addition, the term "Myc-tag" can refer to a single or double Myc-tag. To be on the safe side, we generally recommend elution with the 2x Myc-peptide.
Only for research applications, not for diagnostic or therapeutic use!