Myc-peptide (1x)

Description
Myc-peptide, c-Myc-peptide

Sequence
EQKLISEEDL
corresponds to amino acids 410-419 of the C-terminus of human c-Myc

Applications
Elution of Myc-tagged fusion proteins bound to Myc-Trap® or anti-Myc antibodies, will not elute 3x Myc-tagged proteins.

Note: Optimal dilutions/concentrations should be determined by the user

Product Size Code Price Buy
Product Myc-peptide (1x) Size 1 mg Code yp-1 Price $ 85
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Product Myc-peptide (1x) Size 10 mg Code yp-10 Price $ 640
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Form
lyophilized powder

Size
1 mg; 10 mg

Purity
>96% (HPLC)

Molecular Weight
1.2 kDa

Reconstitution
Reconstitute Myc-peptide in water to a final concentration of 2 mg/ml.

Storage instructions
Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze/ thaw cycles.

How can I gently elute native Myc-tagged protein from the Myc-Trap?

You can elute your Myc-fusion competitively with 1x or 2x Myc peptide. Alternatively, you can use 8 M Urea or 0.2 M glycine pH 2.5 at room temperature.

Should I use 1x Myc or 2x Myc-peptide for elution of the Myc-Trap?

It is likely that the Myc-Trap binds several motifs of a double Myc-tag. Hence, elution of a myc-tagged fusion protein is more efficient with the 2x Myc-peptide. In addition, the term "Myc-tag" can refer to a single or double Myc-tag. To be on the safe side, we generally recommend elution with the 2x Myc-peptide.

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Only for research applications, not for diagnostic or therapeutic use!