corresponds to amino acids 410-419 of the C-terminus of human c-Myc
Elution of Myc-tagged fusion proteins bound to Myc-Trap® or anti-Myc antibodies, will not elute 3x Myc-tagged proteins.
Note: Optimal dilutions/concentrations should be determined by the user
|Product Myc-peptide (1x)||Size 1 mg||Code yp-1||Price $ 85||Buy +|
|Product Myc-peptide (1x)||Size 10 mg||Code yp-10||Price $ 640||Buy +|
1 mg; 10 mg
Reconstitute Myc-peptide in water to a final concentration of 2 mg/ml.
Shipped at ambient temperature. Upon receipt store at -20°C. Aliquot upon reconstitution and store at -20°C. Avoid repeated freeze/ thaw cycles.
How can I gently elute native Myc-tagged protein from the Myc-Trap?
You can elute your Myc-fusion competitively with 1x or 2x Myc peptide. Alternatively, you can use 8 M Urea or 0.2 M glycine pH 2.5 at room temperature.
Should I use 1x Myc or 2x Myc-peptide for elution of the Myc-Trap?
It is likely that the Myc-Trap binds several motifs of a double Myc-tag. Hence, elution of a myc-tagged fusion protein is more efficient with the 2x Myc-peptide. In addition, the term "Myc-tag" can refer to a single or double Myc-tag. To be on the safe side, we generally recommend elution with the 2x Myc-peptide.
Only for research applications, not for diagnostic or therapeutic use!