MK2 VHH
1
Description
Alpaca anti-MK2 VHH, purified recombinant binding protein
Synonyms
MK2-Binding-Protein, MK2-Nanobody, and MK2-single domain antibody (sdAb)
Specificity
MK2, tested on human, mouse, hamster
Posttranslational Modifications: MK2 VHH recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.
Applications
Coupling of biotin, etc. via NHS Ester reaction
| Product | Size | Code | Price | Buy |
|---|---|---|---|---|
| Product MK2 VHH, recombinant binding protein | Size 250 µL | Code mt-250 | Price Please inquire |
Specificity
MK2
Species-Reactivity: tested on human, mouse, hamster
Conjugation:
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Biophysical properties
Molecular weight MW: 14.4 kDa
Molecular extinction coefficient ε: 32,555 L·Mol−1·cm−1
Isoelectrical point pI: 9.7 (theoretical)
Form
Purified recombinant antibody fragment, monoclonal
Protein concentration
1 mg/mL
Storage buffer
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage instructions
Shipped on blue ice. Upon receipt store at 4°C; stable for one year.
RRID
AB_2631377
Do not freeze.
Are the nanobodies monoclonal or polyclonal?
The nanobodies are recombinant/monoclonal.
What are the biophysical parameters of the Nanobodies?
| Name | MW (kDa) | Molar Extinction Coefficient (M-1 cm-1) | Theoretical pI |
|---|---|---|---|
| GFP VHH | 13.9 | 27,055 | 9.4 |
| GST VHH | 14.8 | 28,545 | 8.2 |
| Halo VHH | 14.8 | 23,045 | 9.4 |
| Histon VHH | 14.4 | 25,565 | 10.2 |
| MBP VHH | 14.1 | 27,055 | 10.0 |
| Mdm4/Hdmx VHH | 13.6 | 14,565 | 10.1 |
| MK2 VHH | 14.4 | 32,555 | 9.7 |
| mNeonGreen VHH | 14.3 | 21,680 | 8.0 |
| Myc VHH | 13.5 | 23,045 | 9.6 |
| p53 C-term VHH | 14.8 | 34,170 | 9.2 |
| p53 N-term VHH | 13.0 | 20,065 | 9.7 |
| PARP1 VHH | 14.4 | 17,670 | 9.2 |
| RFP VHH | 14.9 | 30,035 | 9.3 |
| SNAP/CLIP-tag VHH | 13.1 | 24,075 | 9.8 |
| Spot VHH (bivalent) | 30.3 | 46,340 | 5.3 |
| TurboGFP VHH | 13.6 | 18,575 | 9.5 |
| V5 VHH | 14.6 | 22,710 | 8.0 |
| Vimentin VHH | 26.7 | 48,150 | 10.0 |
Do the nanobodies have any tags?
Yes, the nanobodies have a C-terminal His6-tag.
Related products
Only for research applications, not for diagnostic or therapeutic use!