MK2-Trap

Description
The ChromoTek MK2-Trap is a ready to use resin for immunoprecipitation of MK2 fusion proteins.
The MK2-Trap consists of a MK2 Nanobody /VHH coupled to agarose beads.

Specificity
human, mouse, hamster
Posttranslational Modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.

Applications
Immunoprecipitation (IP) / Co-IP
Mass spectrometry (MS)
On-bead enzyme assays

Formats/matrices
MK2-Trap Agarose
MK2 VHH, recombinant binding protein

Synonyms
anti-MK2 VHH, MK2-binding protein, MK2 Nanobody or anti-MK2 single domain antibody fragment (sdAb)

Benefits

  • Pull-down of MK2
  • No heavy & light antibody chains in your downstream application
  • short incubation time (1 h at 4°C)

Format/matrices & properties

About MK2

MK2 (MAP kinase-activated protein kinase 2, MAPKAP-K2) is a stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response, and transcriptional regulation.

Customer feedback

"I was using the MK2 Trap beads to enrich significant amounts of endogenous MK2 to identify isoforms of the kinase. The Trap beads performed so well and specific that I was able to detect them even by Coomassie staining from a low amount of input. The usage was easy and fast forward. The MK2 Trap beads allowed me to study these isoforms in great detail and kick-started the project. "

Dr. Christopher Tiedje, University of Copenhagen

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Only for research applications, not for diagnostic or therapeutic use!