ChromoTek MBP-Trap Agarose beads

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

Specificity

MBP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat no : mbta

Synonyms

MBP, Maltose binding protein, MBP-Trap



Product Information

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

DescriptionImmunoprecipitation of MBP-fusion proteins and their interacting factors with anti-MBP Nanobody conjugated to beads.

• Immunoprecipitation of low abundant MBP-fusion proteins

• Significantly higher sensitivity than amylose resin and other affinity resins

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetMaltose-binding protein (MBP) of Escherichia coli
Binding capacity45 μg (0.625 nmol) of recombinant MBP-tagged protein (~74.2 kDa) per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 4 nM
Compatibility with mass spectrometryTheMBP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631382
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
IP

Mol Cell

Centromere/kinetochore is assembled through CENP-C oligomerization

Authors - Masatoshi Hara

Mol Cell

A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.

Authors - Jiao Zhao
IP

Dev Cell

Importin-β Directly Regulates the Motor Activity and Turnover of a Kinesin-4.

Authors - Anindya Ganguly
IP

Plant Cell

FRA1 Kinesin Modulates the Lateral Stability of Cortical Microtubules through Cellulose Synthase-Microtubule Uncoupling Proteins.

Authors - Anindya Ganguly
IP

Plant Cell

TTL Proteins Scaffold Brassinosteroid Signaling Components at the Plasma Membrane to Optimize Signal Transduction in Arabidopsis.

Authors - Vítor Amorim-Silva
IP

Curr Biol

Arabidopsis pavement cell shape formation involves spatially confined ROPGAP regulators.

Authors - Theresa Lauster