Histone VHH, recombinant binding protein

Description
Alpaca anti-Histone VHH, purified protein, unconjugated

Synonyms
Histone-Binding-Protein, Histone-Nanobody, and Histone-single domain antibody (sdAb)

Specificity
Histone-Label immunostains chromatin throughout the cell cycle (interphase chromatin, chromatin condensation and mitotic chromosomes) due to specific binding to histone H2A-H2B heterodimers. IF performance was validated on cultured human HeLa cells.

Applications
Immunofluorescence
Coupling of dyes, biotin, etc. via NHS Ester reaction

Product Size Code Price Buy
Product Histone VHH, recombinant binding protein Size 250 µL Code tt-250 Price $ 275
Buy +

Specificity
Histone H2A-H2B heterodimers

Conjugation:
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction

Biophysical properties
Molecular weight MW: 14.4 kDa
Molecular extinction coefficient ε: 25,565 L·Mol−1·cm−1
Isoelectrical point pI: 10.2 (theoretical)

Form
Purified recombinant antibody fragment, monoclonal

Protein concentration
1 mg/mL

Storage buffer
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide

Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.

Do not freeze.

Are the nanobodies monoclonal or polyclonal?

The nanobodies are recombinant/monoclonal.

What are the biophysical parameters of the Nanobodies?

Name MW (kDa) Molar Extinction Coefficient
(M-1 cm-1)
Theoretical pI
GFP VHH 13.9 27.055 9.4
GST VHH 14.8 28.545 8.2
Halo VHH 14.8 23.045 9.4
Histon VHH 14.4 25.565 10.2
MBP VHH 14.1 27.055 10.0
Mdm4/Hdmx VHH 13.6 14.565 10.1
MK2 VHH 14.4 32.555 9.7

mNeonGreen VHH

14.3 21.680 8.0
Myc VHH 13.5 23.045 9.6
p53 C-term VHH 14.8 34.170 9.2
p53 N-term VHH 13.0 20.065 9.7
PARP1 VHH 14.4 17.670 9.2
RFP VHH 14.9 30.035 9.3
SNAP/CLIP-tag VHH 13.1 24.075 9.8
Spot VHH (bivalent) 30.3 46.340 5.3
TurboGFP VHH 13.6 18.575 9.5
Vimentin VHH 26.7 48.150 10.0

Do the nanobodies have any tags?

Yes, the nanobodies have a C-terminal His6-tag.

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Only for research applications, not for diagnostic or therapeutic use!