DNA plasmid encoding for anti-H2A-H2B VHH (anti-H2A-H2B Nanobody) fused to EGFP
Histone H2A-H2B heterodimer, tested in mammalian cells, flies, and yeast
Non-invasive live cell imaging
Monitors chromatin dynamics
Histone and chromatin as biomarker
Histone-Chromobody plasmid (EGFP)
|Product Histone-Chromobody® plasmid (EGFP)||Size 20 µg||Code tcg||Price $ 999||Buy +|
constitutive CMV IE
Nuclear localization signal (NLS)
Histone H2A-H2B heterodimer, tested in mammalian cells (HeLa, U2OS, HEK293T, BHK), yeast & flies
Encoded Nanobody/ VHH
Monoclonal anti-H2A-H2B single domain antibody (sdAb) fragment
With the Histone-Chromobody plasmid you receive the sequence information of the alpaca antibody to H2A-H2B heterodimer fused to EGFP and the full vector sequence.
Wide-field epifluorescence microscopy; confocal microscopy
Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Shipped at ambient temperature. Store at -20 °C.
Are Chromobodies constitutively expressed?
Yes, Chromobody expression is regulated by immediate early promotor CMV. This promotor allows constitutive Chromobody expression.
Do Chromobodies only work in live cells?
Yes, the Chromobody plasmid is only expressed in live cells. Cells should be transfected with the Chromobody plasmid at least overnight to observe the Chromobody location signal. Alternatively, cells can be fixed prior to imaging.
Note: We don't recommend fixation of cells for the Histone-Chromobody.
When should I image my cells after transfection with the Chromobody plasmid?
The Chromobody signal is maintained up to 3 days in the cell. However, this also depends strongly on the cell type.
We recommend to image the cells 16-24 hours after transfection.
Do the Chromobodies diffuse through the cell membrane into growth medium?
No, Chromobodies are small proteins being expressed in the cytosol. They are not secreted into the medium and remain in the cell as long as the cell maintains its plasma membrane integrity.
Are Chromobodies fluorogenic or do they only emit fluorescence when bound to a target?
Chromobodies are chimeric proteins consisting of a VHH fused to a fluorescent protein. They maintain their fluorescence regardless of whether they are bound to a target or not.
Can I amplify the Chromobody plasmid in bacteria?
Yes, the Chromobody plasmids can be propagated in E.coli by standard techniques.
Only for research applications, not for diagnostic or therapeutic use!