GFP VHH
Description
Alpaca anti-GFP VHH, purified recombinant binding protein
Synonyms
GFP-Binding-Protein, GFP-Nanobody, and GFP-single domain antibody (sdAb)
Specificity
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-derivatives such as EGFP, CFP, YFP, BFP, and many more. For a full list, see
Fluorescent protein specificity table
Applications
ELISA
Immunofluorescence
Immunoprecipitation
| Product | Size | Code | Price | Buy |
|---|---|---|---|---|
| Product GFP VHH, recombinant binding protein | Size 250 µL | Code gt-250 | Price $ 275 |
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Affinity
Dissociation constant KD of 1 pM
Specificity
GFP, EGFP, CFP, YFP, BFP and many more derivatives of GFP from Aequorea victoria
For Immunoprecipitation see Fluorescent protein specificity table
For Immunofluorescence: CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
Conjugation:
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Biophysical properties
Molecular weight MW: 13.9 kDa
Molecular extinction coefficient ε: 27,055 L·Mol−1·cm−1
Isoelectrical point pI: 9.4 (theoretical)
Form
Purified recombinant antibody fragment, monoclonal
Protein concentration
1 mg/mL
Storage buffer
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
RRID
AB_2631361
Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.
Do not freeze.
Are the Nanobodies monoclonal or polyclonal?
The Nanobodies are recombinant/monoclonal.
What are the biophysical parameters of the Nanobodies?
| Name | MW (kDa) | Molar Extinction Coefficient (M-1 cm-1) | Theoretical pI |
|---|---|---|---|
| GFP VHH | 13.9 | 27,055 | 9.4 |
| GST VHH | 14.8 | 28,545 | 8.2 |
| Halo VHH | 14.8 | 23,045 | 9.4 |
| Histon VHH | 14.4 | 25,565 | 10.2 |
| MBP VHH | 14.1 | 27,055 | 10.0 |
| Mdm4/Hdmx VHH | 13.6 | 14,565 | 10.1 |
| MK2 VHH | 14.4 | 32,555 | 9.7 |
| mNeonGreen VHH | 14.3 | 21,680 | 8.0 |
| Myc VHH | 13.5 | 23,045 | 9.6 |
| p53 C-term VHH | 14.8 | 34,170 | 9.2 |
| p53 N-term VHH | 13.0 | 20,065 | 9.7 |
| PARP1 VHH | 14.4 | 17,670 | 9.2 |
| RFP VHH | 14.9 | 30,035 | 9.3 |
| SNAP/CLIP-tag VHH | 13.1 | 24,075 | 9.8 |
| Spot VHH (bivalent) | 30.3 | 46,340 | 5.3 |
| TurboGFP VHH | 13.6 | 18,575 | 9.5 |
| V5 VHH | 14.6 | 22,710 | 8.0 |
| Vimentin VHH | 26.7 | 48,150 | 10.0 |
Do the Nanobodies have any tags?
Yes, the Nanobodies have a C-terminal His6-tag.
Does the GFP VHH bind to protein A or protein G?
The GFP VHH binds to protein A, but not to protein G.
Extraordinary stable & reliable binding
- GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate
- Fulfills highest requirements for antibody validation
- Structure and function are characterized
Only for research applications, not for diagnostic or therapeutic use!