Alpaca anti-GFP VHH, purified recombinant binding protein

GFP-Binding-Protein, GFP-Nanobody, and GFP-single domain antibody (sdAb)

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-derivatives such as EGFP, CFP, YFP, BFP, and many more. For a full list, see
Fluorescent protein specificity table


Product Size Code Price Buy
Product GFP VHH, recombinant binding protein Size 250 µL Code gt-250 Price $ 275
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Dissociation constant KD of 1 pM

GFP, EGFP, CFP, YFP, BFP and many more derivatives of GFP from Aequorea victoria
For Immunoprecipitation see Fluorescent protein specificity table

For Immunofluorescence: CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine

Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction

Biophysical properties
Molecular weight MW: 13.9 kDa
Molecular extinction coefficient ε: 27,055 L·Mol−1·cm−1
Isoelectrical point pI: 9.4 (theoretical)

Purified recombinant antibody fragment, monoclonal

Protein concentration
1 mg/mL

Storage buffer
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide


Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.

Do not freeze.

Are the Nanobodies monoclonal or polyclonal?

The Nanobodies are recombinant/monoclonal.

What are the biophysical parameters of the Nanobodies?

Name MW (kDa) Molar Extinction Coefficient
(M-1 cm-1)
Theoretical pI
GFP VHH 13.9 27,055 9.4
GST VHH 14.8 28,545 8.2
Halo VHH 14.8 23,045 9.4
Histon VHH 14.4 25,565 10.2
MBP VHH 14.1 27,055 10.0
Mdm4/Hdmx VHH 13.6 14,565 10.1
MK2 VHH 14.4 32,555 9.7
mNeonGreen VHH 14.3 21,680 8.0
Myc VHH 13.5 23,045 9.6
p53 C-term VHH 14.8 34,170 9.2
p53 N-term VHH 13.0 20,065 9.7
PARP1 VHH 14.4 17,670 9.2
RFP VHH 14.9 30,035 9.3
SNAP/CLIP-tag VHH 13.1 24,075 9.8
Spot VHH (bivalent) 30.3 46,340 5.3
TurboGFP VHH 13.6 18,575 9.5
V5 VHH 14.6 22,710 8.0
Vimentin VHH 26.7 48,150 10.0

Do the Nanobodies have any tags?

Yes, the Nanobodies have a C-terminal His6-tag.

Does the GFP VHH bind to protein A or protein G?

The GFP VHH binds to protein A, but not to protein G.

Extraordinary stable & reliable binding

  • GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate
  • Fulfills highest requirements for antibody validation
  • Structure and function are characterized

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Only for research applications, not for diagnostic or therapeutic use!