GFP VHH, biotinylated recombinant binding protein
1
Description
Alpaca anti-GFP VHH, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.
Synonyms
Biotinylated GFP-Binding-Protein, biotinylated GFP-Nanobody, biotinylated anti-GFP camelid single-domain antibody fragment and biotinylated GFP-single domain antibody (sdAb)
Specificity
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and GFP-derivatives such as EGFP, CFP, YFP, BFP, and many more. For a full list, see
Fluorescent protein specificity table
Applications
ELISA, Surface Plasmon Resonance (SPR), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron microscopy (Immuno EM), coating of multiwell plates
| Product | Size | Code | Price | Buy |
|---|---|---|---|---|
| Product GFP VHH, biotinylated recombinant binding protein | Size 250 µL | Code gtb-250 | Price $ 500 |
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Affinity
Dissociation constant KD of 1 pM (binding to GFP)
Specificity
GFP, EGFP, CFP, YFP, BFP and many more derivatives of GFP from Aequorea victoria
For Immunoprecipitation see: Fluorescent protein specificity table
For Immunofluorescence: CFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
Conjugation
Conjugated to biotin (PEG4-Biotin)
Form
Buffered solution
Protein concentration
1 g/ L
Storage buffer
Buffered aqueous solution (25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA), Preservative: 0.09% Sodium azide
Storage instructions
Shipped at ambient temperature. Store at +4°C / 40°F. Stable for one year.
Do not freeze.
Are the Nanobodies monoclonal or polyclonal?
The Nanobodies are recombinant/monoclonal.
What are the biophysical parameters of the Nanobodies?
| Name | MW (kDa) | Molar Extinction Coefficient (M-1 cm-1) | Theoretical pI |
|---|---|---|---|
| GFP VHH | 13.9 | 27.055 | 9.4 |
| GST VHH | 14.8 | 28.545 | 8.2 |
| Halo VHH | 14.8 | 23.045 | 9.4 |
| Histon VHH | 14.4 | 25.565 | 10.2 |
| MBP VHH | 14.1 | 27.055 | 10.0 |
| Mdm4/Hdmx VHH | 13.6 | 14.565 | 10.1 |
| MK2 VHH | 14.4 | 32.555 | 9.7 |
mNeonGreen VHH | 14.3 | 21.680 | 8.0 |
| Myc VHH | 13.5 | 23.045 | 9.6 |
| p53 C-term VHH | 14.8 | 34.170 | 9.2 |
| p53 N-term VHH | 13.0 | 20.065 | 9.7 |
| PARP1 VHH | 14.4 | 17.670 | 9.2 |
| RFP VHH | 14.9 | 30.035 | 9.3 |
| SNAP/CLIP-tag VHH | 13.1 | 24.075 | 9.8 |
| Spot VHH (bivalent) | 30.3 | 46.340 | 5.3 |
| TurboGFP VHH | 13.6 | 18.575 | 9.5 |
| Vimentin VHH | 26.7 | 48.150 | 10.0 |
Do the Nanobodies have any tags?
Yes, the Nanobodies have a C-terminal His6-tag.
Does the GFP VHH bind to protein A or protein G?
The GFP VHH binds to protein A, but not to protein G.
Extraordinary stable & reliable binding
- GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate
- Fulfills highest requirements for antibody validation
- Structure and function are characterized
Only for research applications, not for diagnostic or therapeutic use!