EGFP, recombinant protein
2
Description
Purified recombinant Enhanced Green Fluorescent Protein (EGFP); full length protein; fused to 6xHis-tag
Applications
- Immunoprecipitation: use ~5 µg EGFP per 25 µL GFP-Trap® bead slurry
- SDS-PAGE: use ~ 1 µg EGFP per lane
- Western blot: use ~25 ng - 100 ng EGFP per lane
- ELISA: coat ~ 1ng - 50 ng per well of a 96-well plate
- General: as a fluorescent standard and for calibration
| Product | Size | Code | Price | Buy |
|---|---|---|---|---|
| Product EGFP, recombinant purified protein | Size 250 µg | Code EGFP-250 | Price $ 275 |
Buy
+
|
Form
Liquid; buffered aqueous solution; protein concentration 1g/L
Size
250 µL
Purity
> 80% by SDS-PAGE
Origin
Engineered from Aequorea victoria
Sequence
MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKLEHHHHHH
Molecular Weight
28.0 kDa
Preservative
0.09% sodium azide
Storage instructions
Shipped at +4°C. Store at +4°C short term (up to 1 week). Store at -20°C or -80°C long term. Avoid freeze-thaw cycles. Stable for one year.
Is it possible to elute bound proteins from GFP-Trap® with free GFP?
You may try to elute with free GFP. However, please be aware that this method will not quantitatively elute your fusion protein of interest.
Only for research applications, not for diagnostic or therapeutic use!