PCNA antibody [16D10]
Rat monoclonal [16D10] to Proliferating cell nuclear antigen (PCNA)
Specificity: 16D10 recognizes PCNA from Human, Mouse, Rat, Hamster. Due to sequence homology 16D10 is predicted to react with Cow, Dog, Sheep.
Western Blot: 1/2000
ELISA: not tested
Note: Optimal dilutions can vary and should be determined by the end user
Form: Hybridoma supernatant
Protein concentration: 1,0mg/ml
Preservative: 0.09% sodium azide
Shipped on blue ice. Upon receipt store at 4°C; stable
Intracellular fluorescent antibody for Cell Cycle monitoring in live cells.
- Rottach, A. Kremmer, E. Nowak, D. Boisguerin, P. Volkmer, R. Cardoso, M. C. Leonhardt, H. Rothbauer, U. (2008). Generation and characterization of a rat monoclonal antibody specific for PCNA. Hybridoma (Larchmt). 27: 91-8. (PubMed)
- Spada, F. Haemmer, A. Kuch, D. Rothbauer, U. Schermelleh, L. Kremmer, E. Carell, T. Langst, G. Leonhardt, H. (2007). DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells. J Cell Biol. 176: 565-71. (PubMed)
- Schermelleh, L. Haemmer, A. Spada, F. Rosing, N. Meilinger, D. Rothbauer, U. Cardoso, M. C. Leonhardt, H. (2007). Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation. Nucleic Acids Res. 35: 4301-12. (PubMed)