PCNA antibody [16D10]

Description:
Rat monoclonal [16D10] to Proliferating cell nuclear antigen (PCNA)

Specificity: 16D10 recognizes PCNA from Human, Mouse, Rat, Hamster. Due to sequence homology 16D10 is predicted to react with Cow, Dog, Sheep.

Isotype: IgG2b 

Applications:
Western Blot: 1/2000
Immunofluorescence: 1/2000
ELISA: not tested
Immunoprecipitation: 2.5μl/IP

Note: Optimal dilutions can vary and should be determined by the end user

Form: Hybridoma supernatant

Size: 100μl

Protein concentration: 1,0mg/ml

Preservative: 0.09% sodium azide

Storage instructions:
Shipped on blue ice. Upon receipt store at 4°C; stable 

       

Ordering information

Prices in EUR

SizeCodePriceBuy
25µl16d10-2580 EUR Cart
100µl16d10-100235 EUR Cart

Prices in USD

SizeCodePriceBuy
25µl16d10-25115 USD Cart
100µl16d10-100315 USD Cart
Protocol, SDS, etc.
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References
  • Rottach, A. Kremmer, E. Nowak, D. Boisguerin, P. Volkmer, R. Cardoso, M. C. Leonhardt, H. Rothbauer, U. (2008). Generation and characterization of a rat monoclonal antibody specific for PCNA. Hybridoma (Larchmt). 27: 91-8. (PubMed)

  • Spada, F. Haemmer, A. Kuch, D. Rothbauer, U. Schermelleh, L. Kremmer, E. Carell, T. Langst, G. Leonhardt, H. (2007). DNMT1 but not its interaction with the replication machinery is required for maintenance of DNA methylation in human cells. J Cell Biol. 176: 565-71. (PubMed)

  • Schermelleh, L. Haemmer, A. Spada, F. Rosing, N. Meilinger, D. Rothbauer, U. Cardoso, M. C. Leonhardt, H. (2007). Dynamics of Dnmt1 interaction with the replication machinery and its role in postreplicative maintenance of DNA methylation. Nucleic Acids Res. 35: 4301-12. (PubMed)