Datasheets, protocols & manuals
Antibodies: IgGs
GFP antibody [3H9] datasheet (PDF)
GFP antibody rabbit polyclonal [PABG1] datasheet (PDF)
GST antibody [6G9] datasheet (PDF)
HA antibody [7C9] datasheet (PDF)
mNeonGreen antibody [32F6] datasheet (PDF)
Myc-tag antibody [9E1] datasheet (PDF)
RFP antibody [5F8] datasheet (PDF)
Antibodies: Nanobodies / VHHs
Chromobodies
Actin-Chromobody plasmids
Actin-Chromobody® TagGFP datasheet (PDF)
Actin-Chromobody® TagRFP datasheet (PDF)
Cell Cycle-Chromobody plasmid
Cell Cycle Chromobody® TagRFP datasheet (PDF)
Cell Cycle-Chromobody HeLa cell lines
Cell Cycle-Chromobody® TagGFP HeLa cell line Datasheet (PDF)
Cell Cycle-Chromobody® TagRFP HeLa cell line Datasheet (PDF)
Cell Cycle-Chromobody U2OS cell line
Cell Cycle-Chromobody® TagGFP U2OS cell line Datasheet (PDF)
Cell Cycle-Chromobody® TagRFP U2OS cell line Datasheet (PDF)
Dnmt1-Chromobody Plasmids
Dnmt1-Chromobody® TagGFP datasheet (PDF)
Dnmt1-Chromobody® TagRFP datasheet (PDF)
Histone-Chromobody
Histone-Chromobody® EGFP datasheet (PDF)
Lamin-Chromobody plasmid
Lamin-Chromobody® TagGFP datasheet (PDF)
Lamin-Chromobody HeLa cell lines
HeLa_NMC_TagGFP datasheet (PDF)
HeLa_NMC_TagRFP datasheet (PDF)
Lamin-Chromobody U2OS cell line
U2OS_NMC_TagGFP datasheet (PDF)
PARP1-Chromobody Plasmids
PARP1-Chromobody® TagGFP datasheet (PDF)
PARP1-Chromobody® TagRFP datasheet (PDF)
Vimentin-Chromobody Plasmid
Vimentin-Chromobody® TagGFP datasheet (PDF)
F2H Cellular assays (copy 1)
Fluorescent Two-Hybrid (F2H) Androgen Receptor Kit
F2H_AR protocol (PDF)
Fluorescent Two-Hybrid (F2H) Basic for analyses of protein-protein interactions in live cells
F2H protocol (PDF)
Fluorescent Two-Hybrid (F2H) p53/Mdm2 Kit
F2H_p53-Mdm2 protocol (PDF)
Fluorescent Two-Hybrid (F2H) p53/Mdm4 Kit
F2H_p53-Mdm4 protocol (PDF)
Nano-Boosters & Nano-Labels
GFP-Boosters
GFP-Booster ATTO488 protocol (PDF)
GFP-Booster ATTO594 protocol (PDF)
GFP-Booster ATTO647N protocol (PDF)
GFP-Booster Alexa Fluor 488 (PDF)
GFP-Booster Alexa Fluor 568 (PDF)
GFP-Booster Alexa Fluor 647 (PDF)
Histone_Label
Histone-Label ATTO488 protocol (PDF)
RFP-Boosters
RFP-Booster ATTO594 protocol (PDF)
RFP-Booster ATTO647N protocol (PDF)
Spot-Label
Spot-Label ATTO488 protocol (PDF)
Spot-Label ATTO594 protocol (PDF)
Vimentin-Label
Vimentin-Label ATTO488 protocol (PDF)
Nano-Caps
Nano-CaptureLigands
Protocol Nano-CaptureLigand human Ig, lambda-LC-specific VHH, biotinylated (PDF)
Protocol Nano-CaptureLigand human IgG/ rabbit IgG, Fc-specific VHH, biotinylated (PDF)
Protocol Nano-CaptureLigand mouse IgG1, Fc-specific VHH, biotinylated (PDF)
Protocol Nano-CaptureLigand mouse IgG2a, Fc-specific VHH, biotinylated (PDF)
Protocol Nano-CaptureLigand mouse IgG2b, Fc-specific VHH, biotinylated (PDF)
Nano-Secondaries
Alpaca anti-mouse IgG
Alpaca anti-mouse IgG1, Alexa Fluor® 488 protocol (PDF)
Alpaca anti-mouse IgG1, Alexa Fluor® 568 protocol (PDF)
Alpaca anti-mouse IgG1, Alexa Fluor® 647 protocol (PDF)
Alpaca anti-mouse IgG2b, Alexa Fluor® 488 protocol (PDF)
Alpaca anti-mouse IgG2b, Alexa Fluor® 568 protocol (PDF)
Alpaca anti-mouse IgG2b, Alexa Fluor® 647 protocol (PDF)
Alpaca anti-mouse IgG3, Alexa Fluor® 647 protocol (PDF)
Alpaca anti-rabbit IgG
Alpaca anti-rabbit IgG, Alexa Fluor® 488 protocol (PDF)
Alpaca anti-rabbit IgG, Alexa Fluor® 568 protocol (PDF)
Alpaca anti-rabbit IgG, Alexa Fluor® 647 protocol (PDF)
Nano-Traps
GFP-Trap Agarose
GFP-Trap Agarose protocol (PDF)
GFP-Trap Agarose Kit protocol (PDF)
GFP-Trap Magnetic Agarose
GFP-Trap Magnetic Agarose protocol (PDF)
GFP-Trap Magnetic Agarose Kit protocol (PDF)
GFP-Trap Dynabeads
GFP-Trap Dynabeads protocol (PDF)
GFP-Trap Dynabeads Kit protocol (PDF)
GFP-Trap Multiwell Plate
GFP-Trap Multiwell Plate protocol (PDF)
iST GFP-Trap Kit
Protocol iST GFP-Trap Kit protocol (PDF)
GST-Trap Agarose
GST-Trap Agarose protocol (PDF)
GST-Trap_Agarose Kit protocol (PDF)
Halo-Trap Agarose
Halo-Trap Agarose protocol (PDF)
Halo-Trap Agarose Kit protocol (PDF)
MBP-Trap Agarose
MBP-Trap Agarose protocol (PDF)
Mdm4/HdmX-Trap Agarose
Mdm4/HdmX-Trap Agarose protocol (PDF)
Mdm4/HdmX-Trap Agarose Kit protocol (PDF)
MK2-Trap Agarose
MK2-Trap Agarose protocol (PDF)
MK2-Trap Agarose Kit protocol (PDF)
mNeonGreen-Trap Agarose
mNeonGreen-Trap Agarose protocol (PDF)
mNeonGreen-Trap Agarose Kit protocol (PDF)
mNeonGreen-Trap Magnetic Agarose
mNeonGreen-Trap Magnetic Agarose protocol (PDF)
mNeonGreen-Trap Magnetic Agarose Kit protocol (PDF)
iST mNeonGreen-Trap Kit
Protocol iST mNeonGreen-Trap Kit protocol (PDF)
Myc-Trap Agarose
Myc-Trap Agarose protocol (PDF)
Myc-Trap Agarose Kit protocol (PDF)
Myc-Trap Magnetic Agarose
Myc-Trap Magnetic Agarose protocol (PDF)
Myc-Trap Magnetic Agarose Kit protocol (PDF)
iST Myc-Trap Kit
Protocol iST Myc-Trap Kit protocol (PDF)
p53-C-term-Trap Agarose
p53-C-term-Trap Agarose protocol (PDF)
p53-C-term-Trap Agarose Kit protocol (PDF)
p53-N-term-Trap Agarose
p53-N-term-Trap Agarose protocol (PDF)
p53-N-term-Trap Agarose Kit protocol (PDF)
PARP1-Trap Agarose
PARP1-Trap Agarose protocol (PDF)
PARP1-Trap Agarose Kit protocol (PDF)
RFP-Trap Agarose
RFP-Trap Agarose protocol (PDF)
RFP-Trap Agarose Kit protocol (PDF)
RFP-Trap Magnetic Agarose
RFP-Trap Magnetic Agarose protocol (PDF)
RFP-Trap Magnetic Agarose Kit protocol (PDF)
RFP-Trap Dynabeads
RFP-Trap Dynabeads protocol (PDF)
RFP-Trap Dynabeads Kit protocol (PDF)
iST RFP-Trap Kit
Protocol iST RFP-Trap Kit protocol (PDF)
SNAP/CLIP-tag-Trap Agarose
SNAP/CLIP-tag-Trap Agarose protocol (PDF)
SNAP/CLIP-tag-Trap Agarose Kit protocol (PDF)
Spot-Trap Agarose
Spot-Trap Agarose protocol (PDF)
Spot-Trap Agarose Kit protocol (PDF)
Spot-Trap Magnetic Agarose
Spot-Trap Magnetic Agarose protocol (PDF)
Spot-Trap Magnetic Agarose Kit protocol (PDF)
Spot-Trap Dynabeads
Spot-Trap Dynabeads protocol (PDF)
Spot-Trap Dynabeads Kit protocol (PDF)
iST Spot-Trap Kit
Protocol iST Spot-Trap Kit protocol (PDF)
TurboGFP-Trap Agarose
TurboGFP-Trap Agarose protocol (PDF)
TurboGFP-Trap Agarose Kit protocol (PDF)
TurboGFP-Trap Magnetic Agarose
TurboGFP-Trap Magnetic Agarose protocol (PDF)
TurboGFP-Trap Magnetic Agarose Kit protocol (PDF)
iST TurboGFP-Trap Kit
Protocol iST TurboGFP-Trap Kit protocol (PDF)
Peptides & proteins
Spot-Tag Vectors
Spot vectors - positive controls
| Vector | Code | Data- sheet | Sequence file |
|---|---|---|---|
| pSpot-Tag-Actin vector (plasmid) for expression of Spot-Tag ß-actin fusion protein in mammalian cells - positive control | ev-31 | ev-31.pdf | ev-31.gb |
| pSpot2_GFPSpot-Tag vector (plasmid) for expression of GFP-Spot-Tag fusion protein in E. coli - positive control | ev-32 | ev-32.pdf | ev-32.gb |
| pSpot8_GFP-Spot-Tag vector (plasmid) for expression of GFP-Spot-Tag fusion protein in S. cerevisiae - positive control | ev-33 | ev-33.pdf | ev-33.gb |
Spot vectors for cloning
| Vector | Code | Data- sheet | Sequence file |
|---|---|---|---|
| pSpot1 vector, E. coli, Spot-tag N-term., Kan., high expression | ev-1 | ev-1.pdf | ev-1.gb |
| pSpot2 vector, E. coli, Spot-tag C-term., Kan., high expression | ev-2 | ev-2.pdf | ev-2.gb |
| pSpot3 vector, E. coli, Spot-tag C-term., Amp., low expression | ev-3 | ev-3.pdf | ev-3.gb |
| pSpot4 vector, E. coli, Spot-tag N-term., Amp., low expression | ev-4 | ev-4.pdf | ev-4.gb |
| pSpot5 vector, S. cerevisiae, Spot-tag N-term., Leu, CEN, low expression | ev-5 | ev-5.pdf | ev-5.gb |
| pSpot6 vector, S. cerevisiae, Spot-tag C-term., Leu, CEN, low expression | ev-6 | ev-6.pdf | ev-6.gb |
| pSpot7 vector, S. cerevisiae, Spot-tag N-term., Leu, 2µ, high expression | ev-7 | ev-7.pdf | ev-7.gb |
| pSpot8 vector, S. cerevisiae, Spot-tag C-term., Leu, 2µ, high expression | ev-8 | ev-8.pdf | ev-8.gb |