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Datasheets

Spot-Tag Vectors

Spot vectors - positive controls

VectorCodeData-
sheet		Sequence file
pSpot-Tag-Actin vector (plasmid) for expression of
Spot-Tag ß-actin fusion protein in mammalian cells - positive control 		 ev-31  ev-31.pdf  ev-31.gb
pSpot2_GFPSpot-Tag vector (plasmid) for expression of
GFP-Spot-Tag fusion protein in E. coli - positive control		 ev-32  ev-32.pdf  ev-32.gb
pSpot8_GFP-Spot-Tag vector (plasmid) for expression of
GFP-Spot-Tag fusion protein in S. cerevisiae - positive control		 ev-33  ev-33.pdf  ev-33.gb

 

Spot vectors for cloning

VectorCodeData-
sheet		Sequence file
 pSpot1 vector, E. coli, Spot-tag N-term., Kan., high expression ev-1  ev-1.pdf  ev-1.gb 
 pSpot2 vector, E. coli, Spot-tag C-term., Kan., high expression ev-2  ev-2.pdf  ev-2.gb 
 pSpot3 vector, E. coli, Spot-tag C-term., Amp., low expression ev-3  ev-3.pdf  ev-3.gb 
 pSpot4 vector, E. coli, Spot-tag N-term., Amp., low expression ev-4  ev-4.pdf  ev-4.gb 
 pSpot5 vector, S. cerevisiae, Spot-tag N-term., Leu, CEN, low expression  ev-5  ev-5.pdf  ev-5.gb 
 pSpot6 vector, S. cerevisiae, Spot-tag C-term., Leu, CEN, low expression  ev-6  ev-6.pdf  ev-6.gb 
 pSpot7 vector, S. cerevisiae, Spot-tag N-term., Leu, 2µ, high expression ev-7  ev-7.pdf  ev-7.gb 
 pSpot8 vector, S. cerevisiae, Spot-tag C-term., Leu, 2µ, high expression ev-8  ev-8.pdf  ev-8.gb