The protocol can be applied for all kinds of A. thaliana starting material such as seedlings, leaves and inflorescences.

ChIP protocol for A. thaliana using GFP-Trap (PDF)

RFP-Trap for ChIP analysis in transiently expressing a mCherry fusion in mammalian cells.

ChIP with RFP-tagged proteins (PDF)

Spot-Trap and GFP-Trap for capture and Spot-Label for detection of CRISPR’ed Spot-Tag and EGFP.

Spot-System for CRISPR’ed Spot-Tag (PDF)

The GFP-Trap has a very high affinity and specificity. The GFP-Trap enables very effective pulldowns, but requires special protocols for elution of bound GFP-fusion proteins if they are not analyzed on-bead.

Elution of GFP-fusion protein from the GFP-Trap (PDF)

Using the GFP-Trap, GFP-fusion proteins of interest can be efficiently immunoprecipitated from cellular extracts for subsequent assays.

One step purification of GFP-tagged enzymes for enzymatic activity tests (PDF)

Nano-Secondaries can be simultaneously incubated with the primary antibody. One-step immunostaining saves incubation time and reduces washing steps, and hands-on time.

One-step Immunostaining with Nano-Secondaries (PDF)

On-bead digest protocol for mass spectrometry following immunoprecipitation with GFP-Trap, RFP-Trap, Myc-Trap, Spot-Trap, etc..

On-bead digest protocol for mass spectrometry (PDF)

GFP-Trap for Co-IP of GFP-fusion proteins plus their interaction partners and iST technology for sample preparation for MS analysis.

iST GFP-Trap Kit for mass spectrometry (PDF)

Bead-based protein arrays using GFP-V_HH and GST-V_HH for capture of GFP- and GST-fusion proteins and their interacting partners.

GST- & GFP- nanobodies for bead-based protein arrays (PDF)

GFP-V_HH as capture surface reagent for direct immobilization of GFP-fusion proteins in Biacore^TM protein analysis assays.

Capture Surface for Biacore assays (PDF)

GFP-Trap for the analysis of candidate ubiquitinated proteins under harsh conditions.

Ubiquitination of GFP-tagged proteins (PDF)