The protocol can be applied for all kinds of A. thaliana starting material such as seedlings, leaves and inflorescences.
The GFP-Trap has a very high affinity and specificity. The GFP-Trap enables very effective pulldowns, but requires special protocols for elution of bound GFP-fusion proteins if they are not analyzed on-bead.
Using the GFP-Trap, GFP-fusion proteins of interest can be efficiently immunoprecipitated from cellular extracts for subsequent assays.
Nano-Secondaries can be simultaneously incubated with the primary antibody. One-step immunostaining saves incubation time and reduces washing steps, and hands-on time.
On-bead digest protocol for mass spectrometry following immunoprecipitation with GFP-Trap, RFP-Trap, Myc-Trap, Spot-Trap, etc..