The protocol can be applied for all kinds of A. thaliana starting material such as seedlings, leaves and inflorescences.
The GFP-Trap has a very high affinity and specificity. The GFP-Trap enables very effective pulldowns, but requires special protocols for elution of bound GFP-fusion proteins if they are not analyzed on-bead.
Using the GFP-Trap, GFP-fusion proteins of interest can be efficiently immunoprecipitated from cellular extracts for subsequent assays.
This Application Note provides a short protocol for the use of GFP-Trap® Agarose for IPs from Arabidopsis thaliana samples. It is based on more than 70 peer reviewed publications. Please note that the protocol may also serve as inspiration when working with other plants e.g. Nicotiana .
We combined the GFP-Trap® with the fast, sensitive and robust iST sample preparation kit (PreOmics GmbH). The combination of both workflows enables a streamlined sample preparation of immunoprecipitated GFP-fusion proteins for subsequent analysis by mass spectrometry.
Nano-Secondaries can be simultaneously incubated with the primary antibody. One-step immunostaining saves incubation time and reduces washing steps, and hands-on time.
What blocking solution should I use for Nano-Secondaries? Blocking increases image quality and therefore, it is an essential step during the preparation of a sample for immunofluorescence detection.
On-bead digest protocol for mass spectrometry following immunoprecipitation with GFP-Trap, RFP-Trap, Myc-Trap, Spot-Trap, etc..