High-content screening (HCS) comprises multiple cellular assays that are conducted in an automated, parallel manner to analyze a set of different biological parameters in live cells in a high throughput format. It is used in cell biology and drug discovery to find compounds that impact cellular processes, protein-protein interactions, and/or the phenotype of cells. Tested compounds include small molecules, peptides, and RNAi. Assays are usually based on fluorescence imaging. Next to automated microscopes for data acquisition also automated image analysis software is used, because huge data amounts are generated. HCS is also called high-content analysis (HCA) or cellomics.
ChromoTek’s Chromobodies® are fluorescent nanoprobes for real-time visualization of endogenous intracellular structures inside live cells. Chromobodies are single domain antibodies derived from camelids (Nanobodies or VHHs) that are genetically fused to a fluorescent protein for detection. They are expressed intracellularly and bind to their target within the living cell. Using Chromobodies, dynamic cellular changes can be visualized and monitored in real time. This makes them perfect probes for cellular research including High-Content Analysis (HCA (Schorpp et al 2016).
ChromoTek's Fluorescent Two-Hybrid Technology F2H® detects protein-protein interactions (PPIs) in mammalian cells in a fast and simple manner. The direct read-out can be done using a conventional fluorescence microscope.
The F2H assay visualizes interactions of GFP-fusion proteins with RFP- or mCherry-fusion proteins. It can be used for:
- Validation of PPIs in an intracellular environment
- Identification of interacting protein domains
- Screening PPIs in live cells upon treatment with inhibitors or activators