Nano-Traps
Ready-to-use affinity beads for immunoprecipitation
ChromoTek Nano-Traps are the benchmark in immunoprecipitation (IP) and allow for fast and reliable single-band pulldowns of even low-expression proteins. They consist of Nanobodies coupled to a variety of resins including agarose beads, magnetic agarose beads, and magnetic particles (M-270).
Benefits:
- No heavy & light antibody chains
- Stringent washing conditions
- Low host cell protein background
- Very efficient pulldown, no protein in flow through
- High affinity to bind even proteins expressed at low levels
- Short incubation (30-60 min)
- Recombinantly expressed and validated
Nano-Traps can be applied in:
- IP, Co-IP
- Co-IP/MS
- On-bead assays
- ChIP/RIP analysis
- Split-Fluorescent Protein assays
Immunoprecipitation (IP) of a protein of interest (POI) by Nano-Traps (left) compared with conventional antibodies coupled to Protein A/G beads (right) analyzed by SDS-PAGE. When using Nano-Traps. the amount of immunoprecipitated POI is significantly higher and the background is reduced, in contrast to an IP conducted with a conventional antibody. Nano-Traps provide pure fractions of immunoprecipitated POI without contamination of heavy & light antibody chains. M: Marker, B: Bound.
Watch why GFP-Trap® gives the best results for immunoprecipitation
Low background, no extra bands & high specificity will improve your pulldown assay significantly. Alpaca Alice shows how it works.
For the specificity of GFP-Trap®, mNeonGreen-Trap, TurboGFP-Trap, and RFP-Trap®, view our fluorescent protein specificity table.
Articles
Which GFP-Trap should I use for my immunoprecipitation?
The GFP-Trap consists of the ChromoTek anti-GFP Nanobody/ VHH that is coupled to 3 different types of beads or is immobilized in a 96 multiwell plate.
Non visible textThe best anti-GFP antibody for immunoprecipitation
GFP-Trap consists of an anti-GFP VHH (green circle), derived from camelidae (alpaca, llama, camel, dromedaries, etc.) heavy chain antibodies. The anti-GFP...
Non visible textWhy is the Fab-Trap™ so Fab-ulous?
The Fab-Trap™ is an IP reagent based on a Fab-fragment of a conventional antibody that is covalently coupled to Agarose beads. Fab-Traps™ offer several advantages in IP...
Non visible textGreen fluorescent protein (GFP) in plant research
GFP is increasingly being used as a universal reporter in plant biology from the cellular to whole plant level:...
Non visible textMyc-Trap for IP and affinity purification
Myc-Trap’s performance has been optimized for immunoprecipitation (IP/Co-IP) and affinity purification of Myc-tagged proteins...
Non visible textVirus research using GFP
The interaction between the Nanobody and GFP is extraordinarily stable and efficient. This allows using cell lysate originating from many different organisms that require a multitude of different IP conditions...
Non visible textWhich GFP-Trap should I use for my immunoprecipitation?
The GFP-Trap consists of the ChromoTek anti-GFP Nanobody/ VHH that is coupled to 3 different types of beads or is immobilized in a 96 multiwell plate.
Non visible textThe best anti-GFP antibody for immunoprecipitation
GFP-Trap consists of an anti-GFP VHH (green circle), derived from camelidae (alpaca, llama, camel, dromedaries, etc.) heavy chain antibodies. The anti-GFP...
Non visible textWhy is the Fab-Trap™ so Fab-ulous?
The Fab-Trap™ is an IP reagent based on a Fab-fragment of a conventional antibody that is covalently coupled to Agarose beads. Fab-Traps™ offer several advantages in IP...
Non visible textGreen fluorescent protein (GFP) in plant research
GFP is increasingly being used as a universal reporter in plant biology from the cellular to whole plant level:...
Myc-Trap for IP and affinity purification
Myc-Trap’s performance has been optimized for immunoprecipitation (IP/Co-IP) and affinity purification of Myc-tagged proteins...
Virus research using GFP
The interaction between the Nanobody and GFP is extraordinarily stable and efficient. This allows using cell lysate originating from many different organisms that require a multitude of different IP conditions...
Non visible text