ChromoTek GFP VHH, recombinant binding protein

Alpaca anti-GFP VHH, purified recombinant binding protein

Specificity

GFP and GFP derivates

Applications

Conjugation

Type

Nanobody

Conjugate

Unconjugated

Cat no : gt

Synonyms

GFP-Binding-Protein, GFP-Nanobody, and GFP-single domain antibody (sdAb)



Product Information

Alpaca anti-GFP VHH, purified recombinant binding protein

DescriptionAlpaca anti-GFP VHH, purified recombinant binding protein for extraordinary stable & reliable binding

• GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate

• Fulfills highest requirements for antibody validation

• Structure and function are characterized

ApplicationsSuitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Specificity/TargetCFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
ConjugateUnconjugated
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
Molecular Weight13.9 kDa
RRIDAB_2631361
Storage Buffer25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09 % sodium azide
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C; stable for one year. Do not freeze.

Publications

ApplicationTitle
IF

Nature

TORC1 organized in inhibited domains (TOROIDs) regulate TORC1 activity.

Authors - Manoël Prouteau
IF

Cell

Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells.

Authors - Jieru Li

Nat Methods

Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.

Authors - Yongdeng Zhang
IF

Nat Protoc

Use of fluorescence-detected sedimentation velocity to study high-affinity protein interactions.

Authors - Sumit K Chaturvedi

Trends Biotechnol

Nanobody-based products as research and diagnostic tools.

Authors - Thomas De Meyer
IF

Dev Cell

Multimerization and Retention of the Scavenger Receptor SR-B1 in the Plasma Membrane.

Authors - Pedro E Marques