ChromoTek GFP VHH, biotinylated recombinant binding protein

Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.

Specificity

GFP and GFP derivates

Applications

ELISA, SPR

Type

Nanobody

Conjugate

Biotin

Cat no : gtb

Synonyms

Biotinylated GFP-Binding-Protein, biotinylated GFP-Nanobody, biotinylated anti-GFP camelid single-domain antibody fragment and biotinylated GFP-single domain antibody (sdAb)



Product Information

Alpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin. Compatible with avidin, streptavidin and neutravidin reagents.

DescriptionAlpaca anti-GFP VHH, purified recombinant binding protein, conjugated to biotin for extraordinary stable & reliable binding

• GFP-VHH:GFP complex is stable up to 80 °C, 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100, 3 % Deoxycholate

• Fulfills highest requirements for antibody validation

• Structure and function are characterized

ApplicationsELISA
Surface Plasmon Resonance (SPR)
coating of multiwell plates
Specificity/TargetCFP, eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine
ConjugateBiotin (PEG4-Biotin)
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
RRIDAB_2827557
Storage BufferBuffered aqueous solution (25 mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA), Preservative: 0.09% Sodium azide
Storage ConditionShipped at ambient temperature. Store at +4°C / 40°F. Stable for one year. Do not freeze.

Publications

ApplicationTitle
IP

Nature

cAMP binding to closed pacemaker ion channels is non-cooperative.

Authors - David S White

Nat Nanotechnol

Oligomeric organization of membrane proteins from native membranes at nanoscale spatial and single-molecule resolution

Authors - Gerard Walker
BLI

Nat Commun

Targeting the actin nucleation promoting factor WASp provides a therapeutic approach for hematopoietic malignancies.

Authors - Guy Biber

Nat Commun

Single-molecule imaging with cell-derived nanovesicles reveals early binding dynamics at a cyclic nucleotide-gated ion channel.

Authors - Vishal R Patel
IP

J Cell Biol

Aurora B switches relative strength of kinetochore-microtubule attachment modes for error correction.

Authors - Harinath Doodhi
IF

PLoS Pathog

Poxviruses package viral redox proteins in lateral bodies and modulate the host oxidative response.

Authors - Susanna R. Bidgood